Table 1. Degree of Resistance to... | American Society of Hematology

Table 1.

Degree of Resistance to Cytotoxic Drugs and Fas-Mediated Apoptosis

Cell Line	IC₅₀ of Selecting Agent (mol/L)	DOR to	% Fas-Induced	% Decrease
		Drug	Cell Death	in Apoptosis
8226/S	Dox 2.5 × 10⁻⁹	1	62
	MR 9.7 × 10⁻⁸
8226/Dox1	3.2 × 10⁻⁸	13	49	21
8226/Dox6	2.8 × 10⁻⁷	110	21	66
8226/Dox40	2.7 × 10⁻⁶	1,350	12	81
8226/Dox40ood	4.5 × 10⁻⁷	180	18	71
8226/MR4	1.0 × 10⁻⁶	11	42	32
8226/MR20	3.6 × 10⁻⁶	37	19	69
8226/MR20ood	2.8 × 10⁻⁶	30	21	66
CEM/S	3.2 × 10⁻⁸	1	96
CEM/Dox1-F5	2.9 × 10⁻⁹	3	72	25

Cell Line	IC₅₀ of Selecting Agent (mol/L)	DOR to	% Fas-Induced	% Decrease
		Drug	Cell Death	in Apoptosis
8226/S	Dox 2.5 × 10⁻⁹	1	62
	MR 9.7 × 10⁻⁸
8226/Dox1	3.2 × 10⁻⁸	13	49	21
8226/Dox6	2.8 × 10⁻⁷	110	21	66
8226/Dox40	2.7 × 10⁻⁶	1,350	12	81
8226/Dox40ood	4.5 × 10⁻⁷	180	18	71
8226/MR4	1.0 × 10⁻⁶	11	42	32
8226/MR20	3.6 × 10⁻⁶	37	19	69
8226/MR20ood	2.8 × 10⁻⁶	30	21	66
CEM/S	3.2 × 10⁻⁸	1	96
CEM/Dox1-F5	2.9 × 10⁻⁹	3	72	25

Degree of resistance (DOR) is relative to the parent cell line, 8226/S. The DOR is determined by dividing the IC₅₀ for the resistant cell line (8226/Dox, 8226/MR, or CEM/Dox) by the IC₅₀ for the parent cell line.35 Fas-induced cell death is calculated by the percentage of apoptotic cells after treatment with 200 ng/mL of anti-Fas (CH-11) antibody minus the percentage of apoptotic cells in controls treated with mouse IgM. The percentage of decrease in apoptosis due to Fas is determined by

\frac{(% Cell Death in Sensitive Cell Line) - (% Cell Death in Resistant Cell Line)}{(% Cell Death in Sensitive Cell Line)}

Each value represents the average of at least three independent trials.

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