Expected Molecular Masses of Gla-Domain Fragments Obtained by Endoproteinase Asp-N Digestion of Protein C Light Chain
| N-Terminal Amino Acid . | Gla Residues . | C-Terminal Amino Acid . | |
|---|---|---|---|
| . | . | Val34 . | Asp35 . |
| Ala1 | Intact | 4,569 | 4,684 |
| Decarboxylated | 4,173 | 4,288 | |
| His−1 | Intact | 4,706 | 4,821 |
| Decarboxylated | 4,310 | 4,425 | |
| N-Terminal Amino Acid . | Gla Residues . | C-Terminal Amino Acid . | |
|---|---|---|---|
| . | . | Val34 . | Asp35 . |
| Ala1 | Intact | 4,569 | 4,684 |
| Decarboxylated | 4,173 | 4,288 | |
| His−1 | Intact | 4,706 | 4,821 |
| Decarboxylated | 4,310 | 4,425 | |
Values (in Daltons) are given for fragments originating from cleavage before Asp35 and Asp36, respectively. Furthermore values are given for the normal form starting at Ala1 as well as for the mutant extended with His−1.