Identification of Step-Wise Opsonized E Used in Figs 1 Through 4
| E Used . | Step 1 . | Step 2 . | Step 3 . | E Designation . | Experiment . |
|---|---|---|---|---|---|
| Human | Human anti-(D) Rho | MoAb HB43 (antihuman IgG) | — | Human anti-(D) Rho/mouse antihuman IgG | Fig 1 |
| Biotinylated human E | Streptavidin | Biotinylated MoAb 4-15 (antihuman IgE) | — | b-E/SA/b-4-15 | Fig 1 |
| Human | MoAb 7G9 (anti-CR1) | — | — | Anti-CR1 | Fig 1 |
| Human | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated MoAb 4-15 | E/b-7G9/SA/b-4-15 | Fig 1 |
| Human | MoAb E3 (antiglycophorin) | — | — | Antiglycophorin (IgG1) | Fig 2 |
| Human | MoAb 1B4 (anti-CR1) | — | — | Anti-CR1 (1B4, IgG1) | Fig 2 |
| Human | MoAb 7G9 | — | — | Anti-CR1 (7G9, IgG2a) | Fig 2 |
| Human | MoAb 1B4 + MoAb 7G9 | — | — | Anti-CR1 cocktail | Fig 2 |
| Biotinylated rhesus monkey E | BSA | — | — | BSA | Figs 3 & 4 |
| Biotinylated rhesus monkey E | MoAb 7G9 | — | — | 7G9 | Figs 3 & 4 |
| Biotinylated rhesus monkey E | Streptavidin | Biotinylated MoAb 7G9 | — | SA/b-7G9 | Fig 3 |
| Biotinylated rhesus monkey E | MoAb 7G9 | Streptavidin | Biotinylated MoAb 7G9 | 7G9/SA/b-7G9 | Figs 3 & 4 |
| Biotinylated rhesus monkey E | Mouse polyclonal anti-CR1 IgG antibodies | — | — | p@CR1 | Fig 3 |
| Biotinylated rhesus monkey E | Streptavidin | Mouse polyclonal anti-CR1 IgG antibodies | — | SA/p@CR1 | Fig 3 |
| Biotinylated Sheep E* | Streptavidin | Biotinylated MoAb 7G9 | — | SA/b-7G9 | Fig 3 |
| Rhesus monkey E | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated MoAb 4-15 | b-7G9/SA/b-4-15 | Fig 4 |
| Rhesus monkey E | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated mouse IgG | b-7G9/SA/b-msIgG | Fig 4 |
| Rhesus monkey E | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated MoAb 7G9 | b-7G9/SA/b-7G9 | Fig 4 |
| Rhesus monkey E | MoAb 7G9 | — | — | 7G9 | Fig 4 |
| Biotinylated rhesus monkey E | Streptavidin | Biotinylated MoAb 4-15 | — | SA/b-4-15 | Fig 4 |
| Biotinylated rhesus monkey E | Streptavidin | Biotinylated mouse IgG | — | SA/b-msIgG | Fig 4 |
| Biotinylated rhesus monkey E | Streptavidin | MoAb 7G9 | — | SA/7G9 | Fig 4 |
| E Used . | Step 1 . | Step 2 . | Step 3 . | E Designation . | Experiment . |
|---|---|---|---|---|---|
| Human | Human anti-(D) Rho | MoAb HB43 (antihuman IgG) | — | Human anti-(D) Rho/mouse antihuman IgG | Fig 1 |
| Biotinylated human E | Streptavidin | Biotinylated MoAb 4-15 (antihuman IgE) | — | b-E/SA/b-4-15 | Fig 1 |
| Human | MoAb 7G9 (anti-CR1) | — | — | Anti-CR1 | Fig 1 |
| Human | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated MoAb 4-15 | E/b-7G9/SA/b-4-15 | Fig 1 |
| Human | MoAb E3 (antiglycophorin) | — | — | Antiglycophorin (IgG1) | Fig 2 |
| Human | MoAb 1B4 (anti-CR1) | — | — | Anti-CR1 (1B4, IgG1) | Fig 2 |
| Human | MoAb 7G9 | — | — | Anti-CR1 (7G9, IgG2a) | Fig 2 |
| Human | MoAb 1B4 + MoAb 7G9 | — | — | Anti-CR1 cocktail | Fig 2 |
| Biotinylated rhesus monkey E | BSA | — | — | BSA | Figs 3 & 4 |
| Biotinylated rhesus monkey E | MoAb 7G9 | — | — | 7G9 | Figs 3 & 4 |
| Biotinylated rhesus monkey E | Streptavidin | Biotinylated MoAb 7G9 | — | SA/b-7G9 | Fig 3 |
| Biotinylated rhesus monkey E | MoAb 7G9 | Streptavidin | Biotinylated MoAb 7G9 | 7G9/SA/b-7G9 | Figs 3 & 4 |
| Biotinylated rhesus monkey E | Mouse polyclonal anti-CR1 IgG antibodies | — | — | p@CR1 | Fig 3 |
| Biotinylated rhesus monkey E | Streptavidin | Mouse polyclonal anti-CR1 IgG antibodies | — | SA/p@CR1 | Fig 3 |
| Biotinylated Sheep E* | Streptavidin | Biotinylated MoAb 7G9 | — | SA/b-7G9 | Fig 3 |
| Rhesus monkey E | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated MoAb 4-15 | b-7G9/SA/b-4-15 | Fig 4 |
| Rhesus monkey E | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated mouse IgG | b-7G9/SA/b-msIgG | Fig 4 |
| Rhesus monkey E | Biotinylated MoAb 7G9 | Streptavidin | Biotinylated MoAb 7G9 | b-7G9/SA/b-7G9 | Fig 4 |
| Rhesus monkey E | MoAb 7G9 | — | — | 7G9 | Fig 4 |
| Biotinylated rhesus monkey E | Streptavidin | Biotinylated MoAb 4-15 | — | SA/b-4-15 | Fig 4 |
| Biotinylated rhesus monkey E | Streptavidin | Biotinylated mouse IgG | — | SA/b-msIgG | Fig 4 |
| Biotinylated rhesus monkey E | Streptavidin | MoAb 7G9 | — | SA/7G9 | Fig 4 |
In all cases, unless otherwise noted, the E were reacted with rabbit antimouse IgG after the last listed step.
Bar to the right of dashed line in Fig 3.