Expression of WT1 mRNA in FACS-Sorted Cells
| BM | CB | ||||
| Donor No. | CD34+CD33− | CD34+CD33+ | Donor No. | CD34+CD33− | CD34+CD33+ |
| 1 | 2.0 × 10−2 | <10−2 | 18 | <10−2 | <10−2 |
| 2 | <10−2 | <10−2 | 19 | <10−2 | <10−2 |
| 3 | <10−2 | <10−2 | 20 | <10−2 | <10−2 |
| 4 | <10−2 | <10−2 | 21 | 1.5 × 10−2 | <10−2 |
| 5 | <10−2 | <10−2 | |||
| 6 | <10−2 | <10−2 | |||
| BM | CB | ||||
| Donor No. | CD34+CD33− | CD34+CD33+ | Donor No. | CD34+CD33− | CD34+CD33+ |
| 1 | 2.0 × 10−2 | <10−2 | 18 | <10−2 | <10−2 |
| 2 | <10−2 | <10−2 | 19 | <10−2 | <10−2 |
| 3 | <10−2 | <10−2 | 20 | <10−2 | <10−2 |
| 4 | <10−2 | <10−2 | 21 | 1.5 × 10−2 | <10−2 |
| 5 | <10−2 | <10−2 | |||
| 6 | <10−2 | <10−2 | |||
| Donor No. | CD34+CD38− | CD34+CD38+ | Donor No. | CD34+CD38− | CD34+CD38+ |
| 7 | <10−2 | <10−2 | 22 | <10−2 | <10−2 |
| 8 | <10−2 | 1.3 × 10−2 | 23 | <10−2 | 1.0 × 10−2 |
| 24 | 1.3 × 10−2 | <10−2 | |||
| 25 | 1.7 × 10−2 | 1.0 × 10−2 | |||
| Donor No. | CD34+CD38− | CD34+CD38+ | Donor No. | CD34+CD38− | CD34+CD38+ |
| 7 | <10−2 | <10−2 | 22 | <10−2 | <10−2 |
| 8 | <10−2 | 1.3 × 10−2 | 23 | <10−2 | 1.0 × 10−2 |
| 24 | 1.3 × 10−2 | <10−2 | |||
| 25 | 1.7 × 10−2 | 1.0 × 10−2 | |||
| Donor No. | CD34+HLA-DR− | CD34+HLA-DR+ | Donor No. | CD34+HLA-DR− | CD34+HLA-DR+ |
| 9 | 1.3 × 10−2 | 2.2 × 10−2 | 26 | NE | <10−2 |
| 10 | NE | 2.4 × 10−2 | 27 | <10−2 | 1.0 × 10−2 |
| 28 | 1.4 × 10−2 | 2.4 × 10−2 | |||
| Donor No. | CD34+HLA-DR− | CD34+HLA-DR+ | Donor No. | CD34+HLA-DR− | CD34+HLA-DR+ |
| 9 | 1.3 × 10−2 | 2.2 × 10−2 | 26 | NE | <10−2 |
| 10 | NE | 2.4 × 10−2 | 27 | <10−2 | 1.0 × 10−2 |
| 28 | 1.4 × 10−2 | 2.4 × 10−2 | |||
| Donor No. | CD34+c-kithigh | CD34+c-kitlow | CD34+c-kit− | Donor No. | CD34+c-kithigh | CD34+c-kitlow | CD34+c-kit− |
| 11 | <10−2 | <10−2 | <10−2 | 29 | <10−2 | <10−2 | <10−2 |
| 12 | <10−2 | <10−2 | NE | 30 | <10−2 | <10−2 | 1.0 × 10−2 |
| 13 | 1.3 × 10−2 | <10−2 | NE | ||||
| 14 | 1.0 × 10−2 | 1.5 × 10−2 | NE | ||||
| Donor No. | CD34+c-kithigh | CD34+c-kitlow | CD34+c-kit− | Donor No. | CD34+c-kithigh | CD34+c-kitlow | CD34+c-kit− |
| 11 | <10−2 | <10−2 | <10−2 | 29 | <10−2 | <10−2 | <10−2 |
| 12 | <10−2 | <10−2 | NE | 30 | <10−2 | <10−2 | 1.0 × 10−2 |
| 13 | 1.3 × 10−2 | <10−2 | NE | ||||
| 14 | 1.0 × 10−2 | 1.5 × 10−2 | NE | ||||
| Donor No. | CD34+ | CD34− | Donor No. | CD34+ | CD34− |
| 15 | <10−2 | <10−2 | 31 | 1.5 × 10−2 | <10−2 |
| 16 | 1.0 × 10−2 | <10−2 | 32 | 2.4 × 10−2 | <10−2 |
| 17 | 2.2 × 10−2 | <10−2 | |||
| Donor No. | CD34+ | CD34− | Donor No. | CD34+ | CD34− |
| 15 | <10−2 | <10−2 | 31 | 1.5 × 10−2 | <10−2 |
| 16 | 1.0 × 10−2 | <10−2 | 32 | 2.4 × 10−2 | <10−2 |
| 17 | 2.2 × 10−2 | <10−2 | |||
| FACS-Sorted Leukemic Cells . | |||||||
|---|---|---|---|---|---|---|---|
| Patient No. FAB . | CD34 (%) . | CD33 (%) . | Unsorted . | CD34+CD33+ . | CD34+CD33− . | CD34−CD33+ . | CD34−CD33− . |
| 293 | 68 | 58 | 1.5 × 10−1 | 5.4 × 10−1 | 4.8 × 10−1 | 2.9 × 10−1 | 1.6 × 10−1 |
| M1 | |||||||
| 273 | 79 | 92 | 8.9 × 10−1 | 9.9 × 10−1 | 1.9 × 100 | 1.8 × 100 | 5.6 × 10−1 |
| M2 | |||||||
| 194 | 10 | 72 | 6.4 × 10−1 | 3.4 × 10−1 | 8.9 × 10−1 | 3.6 × 10−1 | 8.6 × 10−1 |
| M4 | |||||||
| 294 | 78 | 96 | 4.1 × 10−1 | 3.5 × 10−1 | 4.4 × 10−1 | 2.3 × 10−1 | 5.4 × 10−1 |
| M4 | |||||||
| 271 | 9 | 91 | 1.0 × 10−2 | ND | ND | 7.0 × 10−2 | 1.3 × 10−1 |
| M5b | |||||||
| FACS-Sorted Leukemic Cells . | |||||||
|---|---|---|---|---|---|---|---|
| Patient No. FAB . | CD34 (%) . | CD33 (%) . | Unsorted . | CD34+CD33+ . | CD34+CD33− . | CD34−CD33+ . | CD34−CD33− . |
| 293 | 68 | 58 | 1.5 × 10−1 | 5.4 × 10−1 | 4.8 × 10−1 | 2.9 × 10−1 | 1.6 × 10−1 |
| M1 | |||||||
| 273 | 79 | 92 | 8.9 × 10−1 | 9.9 × 10−1 | 1.9 × 100 | 1.8 × 100 | 5.6 × 10−1 |
| M2 | |||||||
| 194 | 10 | 72 | 6.4 × 10−1 | 3.4 × 10−1 | 8.9 × 10−1 | 3.6 × 10−1 | 8.6 × 10−1 |
| M4 | |||||||
| 294 | 78 | 96 | 4.1 × 10−1 | 3.5 × 10−1 | 4.4 × 10−1 | 2.3 × 10−1 | 5.4 × 10−1 |
| M4 | |||||||
| 271 | 9 | 91 | 1.0 × 10−2 | ND | ND | 7.0 × 10−2 | 1.3 × 10−1 |
| M5b | |||||||
Abbreviations: NE, not able to evaluate because of low levels of β-actin; ND, not determined; FAB, French-American-British criteria.