Correlation Between WT1 Gene Expression Levels and Surface Markers
| Leukemic Samples . | ||||||
|---|---|---|---|---|---|---|
| FAB . | Patient No. . | WT1 mRNA . | CD34 . | CD33 . | CD38 . | HLA-DR . |
| L1 | 289 | 0.93 | + | − | + | − |
| M1 | 80 | 0.57 | + | − | − | + |
| AMLL | 51 | 0.44 | + | − | + | + |
| ALL | 81 | 0.34 | + | − | + | ND |
| ALL | 197 | 0.25 | + | − | − | + |
| M2 | 124 | 0.11 | + | − | ND | + |
| M2 | 237 | 0.074 | + | − | + | + |
| M2 | 220 | 0.022 | + | − | + | ND |
| L2 | 234 | 0.021 | + | − | + | ND |
| L1 | 275 | 0.012 | + | − | − | + |
| M2 | 273 | 0.89 | + | + | ND | − |
| M3 | 228 | 0.84 | + | + | − | − |
| L2 | 139 | 0.70 | + | + | ND | + |
| M1 | 6 | 0.45 | + | + | ND | + |
| M4 | 294 | 0.41 | + | + | + | + |
| MDS-AML | 318 | 0.30 | + | + | + | ND |
| MDS-AML | 243 | 0.20 | + | + | ND | + |
| M4 | 227 | 0.05 | + | + | + | ND |
| ALL | 288 | 0.026 | + | + | + | ND |
| M2 | 199 | 0.026 | + | + | + | + |
| M5a | 37 | 1.72 | − | + | ND | + |
| M1 | 317 | 1.34 | − | + | + | − |
| M3 | 282 | 1.01 | − | + | ND | − |
| M6 | 287 | 0.98 | − | + | + | + |
| M4 | 275 | 0.97 | − | + | + | + |
| M5a | 306 | 0.62 | − | + | + | + |
| M3 | 78 | 0.58 | − | + | ND | − |
| M2 | 235 | 0.45 | − | + | + | − |
| M5 | 296 | 0.11 | − | + | + | + |
| M3 | 137 | 0.025 | − | + | ND | − |
| M5 | 274 | 0.013 | − | + | + | + |
| M5b | 271 | 0.010 | − | + | ND | + |
| L2 | 5 | 0.40 | − | − | + | − |
| ALL | 280 | 0.25 | − | − | + | ND |
| M5a | 33 | 0.050 | − | − | + | + |
| L2 | 151 | 0.017 | − | − | + | + |
| Leukemic Samples . | ||||||
|---|---|---|---|---|---|---|
| FAB . | Patient No. . | WT1 mRNA . | CD34 . | CD33 . | CD38 . | HLA-DR . |
| L1 | 289 | 0.93 | + | − | + | − |
| M1 | 80 | 0.57 | + | − | − | + |
| AMLL | 51 | 0.44 | + | − | + | + |
| ALL | 81 | 0.34 | + | − | + | ND |
| ALL | 197 | 0.25 | + | − | − | + |
| M2 | 124 | 0.11 | + | − | ND | + |
| M2 | 237 | 0.074 | + | − | + | + |
| M2 | 220 | 0.022 | + | − | + | ND |
| L2 | 234 | 0.021 | + | − | + | ND |
| L1 | 275 | 0.012 | + | − | − | + |
| M2 | 273 | 0.89 | + | + | ND | − |
| M3 | 228 | 0.84 | + | + | − | − |
| L2 | 139 | 0.70 | + | + | ND | + |
| M1 | 6 | 0.45 | + | + | ND | + |
| M4 | 294 | 0.41 | + | + | + | + |
| MDS-AML | 318 | 0.30 | + | + | + | ND |
| MDS-AML | 243 | 0.20 | + | + | ND | + |
| M4 | 227 | 0.05 | + | + | + | ND |
| ALL | 288 | 0.026 | + | + | + | ND |
| M2 | 199 | 0.026 | + | + | + | + |
| M5a | 37 | 1.72 | − | + | ND | + |
| M1 | 317 | 1.34 | − | + | + | − |
| M3 | 282 | 1.01 | − | + | ND | − |
| M6 | 287 | 0.98 | − | + | + | + |
| M4 | 275 | 0.97 | − | + | + | + |
| M5a | 306 | 0.62 | − | + | + | + |
| M3 | 78 | 0.58 | − | + | ND | − |
| M2 | 235 | 0.45 | − | + | + | − |
| M5 | 296 | 0.11 | − | + | + | + |
| M3 | 137 | 0.025 | − | + | ND | − |
| M5 | 274 | 0.013 | − | + | + | + |
| M5b | 271 | 0.010 | − | + | ND | + |
| L2 | 5 | 0.40 | − | − | + | − |
| ALL | 280 | 0.25 | − | − | + | ND |
| M5a | 33 | 0.050 | − | − | + | + |
| L2 | 151 | 0.017 | − | − | + | + |
| Leukemia and Lymphoma Cell Lines . | |||||||
|---|---|---|---|---|---|---|---|
| Cell Line . | Origin . | WT1 mRNA . | CD34 . | CD33 . | CD38 . | HLA-DR . | c-kit . |
| Molt-4 | T-ALL | 0.41 | + | − | + | − | − |
| KG-1 | M1 | 1.12 | + | + | − | − | +low |
| KU812 | CML | 0.83 | + | + | + | − | +low |
| TF-1 | M6 | 0.80 | + | + | + | + | +low |
| Kasumi-1 | M2 | 0.60 | + | + | + | + | +low |
| BV173 | CML | 1.50 | − | + | + | + | − |
| K562 | CML | 1.00 | − | + | − | − | − |
| HL-60 | M3 | 0.56 | − | + | + | − | − |
| THP-1 | M5 | 0.45 | − | + | + | − | − |
| HEL | M6 | 0.37 | − | + | − | + | +low |
| IMS-M1 | M5 | 0.31 | − | + | + | − | − |
| SAM-1 | CML | 0.71 | − | − | − | − | − |
| Molt-3 | T-ALL | 0.10 | − | − | + | − | − |
| Tall-1 | T-ALL | 0.04 | − | − | + | − | − |
| Jurkat | T-ALL | 2.0 × 10−4 | − | − | + | − | − |
| Ball-1 | B-ALL | <10−5 | − | − | + | + | ND |
| Daudi | Lymphoma | 3.8 × 10−4 | − | − | + | + | − |
| U937 | Lymphoma | <10−5 | − | + | + | − | − |
| KT-3 | Lymphoma | <10−5 | − | − | − | + | − |
| DL-40 | Lymphoma | <10−5 | − | − | + | + | − |
| Leukemia and Lymphoma Cell Lines . | |||||||
|---|---|---|---|---|---|---|---|
| Cell Line . | Origin . | WT1 mRNA . | CD34 . | CD33 . | CD38 . | HLA-DR . | c-kit . |
| Molt-4 | T-ALL | 0.41 | + | − | + | − | − |
| KG-1 | M1 | 1.12 | + | + | − | − | +low |
| KU812 | CML | 0.83 | + | + | + | − | +low |
| TF-1 | M6 | 0.80 | + | + | + | + | +low |
| Kasumi-1 | M2 | 0.60 | + | + | + | + | +low |
| BV173 | CML | 1.50 | − | + | + | + | − |
| K562 | CML | 1.00 | − | + | − | − | − |
| HL-60 | M3 | 0.56 | − | + | + | − | − |
| THP-1 | M5 | 0.45 | − | + | + | − | − |
| HEL | M6 | 0.37 | − | + | − | + | +low |
| IMS-M1 | M5 | 0.31 | − | + | + | − | − |
| SAM-1 | CML | 0.71 | − | − | − | − | − |
| Molt-3 | T-ALL | 0.10 | − | − | + | − | − |
| Tall-1 | T-ALL | 0.04 | − | − | + | − | − |
| Jurkat | T-ALL | 2.0 × 10−4 | − | − | + | − | − |
| Ball-1 | B-ALL | <10−5 | − | − | + | + | ND |
| Daudi | Lymphoma | 3.8 × 10−4 | − | − | + | + | − |
| U937 | Lymphoma | <10−5 | − | + | + | − | − |
| KT-3 | Lymphoma | <10−5 | − | − | − | + | − |
| DL-40 | Lymphoma | <10−5 | − | − | + | + | − |
Abbreviation: ND, not determined; FAB, French-American-British criteria.