Platelet Adhesion to Collagen Type III Preincubated With rvWFs
| Surface . | % Coverage . |
|---|---|
| WT | 56 ± 2 |
| R543Q | 61 ± 3 (NS) |
| R543W | 50 ± 8 (NS) |
| HAS | 4 ± 0.8* |
| Surface . | % Coverage . |
|---|---|
| WT | 56 ± 2 |
| R543Q | 61 ± 3 (NS) |
| R543W | 50 ± 8 (NS) |
| HAS | 4 ± 0.8* |
Recombinant vWFs (WT, R543Q, and R543W) were coated to glass coverslips, which were preincubated with collagen type III. As a control, human albumin (HAS) was coated to collagen-treated coverslips. The coverslips were exposed to a shear rate of 1,600 s−1 using reconstituted platelets in vWF-depleted citrated plasma as the perfusate. The data are mean ± SEM obtained in three independent experiments each performed in triplicate.
P < .001 compared with WT-vWF.