IL-10 Inhibits Spontaneous CFU-GM Growth From Normal Human PB-MNCs
| Exp. No. . | No. of Colonies . | % Inhibition . | |
|---|---|---|---|
| . | Spontaneous Growth . | +IL-10 . | . |
| 1 | 7.3 | 0.0 | 100 |
| 2 | 9.0 | 1.0 | 88.8 |
| 3 | 11.3 | 0.3 | 97 |
| 4 | 9.6 | 0.0 | 100 |
| 5 | 7.0 | 0.6 | 90.6 |
| 6 | 10.6 | 0.3 | 96.9 |
| 7 | 17.0 | 1.0 | 94.1 |
| Mean ± SEM | 10.3 ± 1.27 | 0.5 ± 0.16 | 95.1* |
| Exp. No. . | No. of Colonies . | % Inhibition . | |
|---|---|---|---|
| . | Spontaneous Growth . | +IL-10 . | . |
| 1 | 7.3 | 0.0 | 100 |
| 2 | 9.0 | 1.0 | 88.8 |
| 3 | 11.3 | 0.3 | 97 |
| 4 | 9.6 | 0.0 | 100 |
| 5 | 7.0 | 0.6 | 90.6 |
| 6 | 10.6 | 0.3 | 96.9 |
| 7 | 17.0 | 1.0 | 94.1 |
| Mean ± SEM | 10.3 ± 1.27 | 0.5 ± 0.16 | 95.1* |
PB-MNCs (2 × 105/mL) were cultured in methylcellulose containing 30% FCS and IMDM without addition of CSF in the presence and absence of IL-10 (10 ng/mL). Colony growth was assessed after 14 days. The mean colony numbers of triplicates are given from seven experiments.
P < .001.