Susceptibility to Recognition and Lysis Mediated by the Autologous GVHD Effector Cells Is Enhanced by Force Loading CLIP
| Pretreatment . | Percentage of Specific Lysis . | |
|---|---|---|
| . | of Treated Target Cells . | |
| . | PHA Blast Cells . | T47D Cells . |
| CLIP loaded (n = 3) | 30.2 ± 3.9 | 34.9 ± 2.7 |
| Control (n = 3) | 15.3 ± 2.1 | 16.4 ± 1.3 |
| Pretreatment . | Percentage of Specific Lysis . | |
|---|---|---|
| . | of Treated Target Cells . | |
| . | PHA Blast Cells . | T47D Cells . |
| CLIP loaded (n = 3) | 30.2 ± 3.9 | 34.9 ± 2.7 |
| Control (n = 3) | 15.3 ± 2.1 | 16.4 ± 1.3 |
Pretransplant PHA-stimulated lymphoblasts or T47D cells labeled with 51Cr were loaded with CLIP (1.0 μmol/L) or an irrelevant peptide for 2 hours at 4°C. The cells were washed three times in RPMI 1640 before use as target cells in a 51Cr release assay. Effector lymphocytes were from patients with biopsy-confirmed autologous GVHD. The effector to target ratio was 100:1.