Regulation of Bcl-2 and Bax in Bcl-2+ ALL Cell Lines After Treatment With IR
| Cell Line . | Downregulation of Bcl-2 . | Upregulation of Bax . | Apoptosis . |
|---|---|---|---|
| Reh | + | − | − |
| EU-1 | + | − | − |
| EU-3 | + | + | + |
| Sup-B13 | + | + | + |
| Sup-B15 | + | + | + |
| Uoc-B1 | − | + | − |
| Uoc-B4 | + | + | + |
| Uoc-B11 | + | + | + |
| EU-6* | − | − | − |
| Cell Line . | Downregulation of Bcl-2 . | Upregulation of Bax . | Apoptosis . |
|---|---|---|---|
| Reh | + | − | − |
| EU-1 | + | − | − |
| EU-3 | + | + | + |
| Sup-B13 | + | + | + |
| Sup-B15 | + | + | + |
| Uoc-B1 | − | + | − |
| Uoc-B4 | + | + | + |
| Uoc-B11 | + | + | + |
| EU-6* | − | − | − |
Expression of Bcl-2 and Bax mRNA was detected by Northern blot analysis after treatment with IR (10 Gy) for 2 to 8 hours. Apoptosis was measured by DNA fragmentation after IR for 5 hours.
EU-6 expressed mutant p53.