Appearance of Cell Surface Antigens Correlated to NBT Positivity
| . | Mock Clone M6 . | Antisense Rb Clone A1 . | ||||
|---|---|---|---|---|---|---|
| . | Control . | ATRA . | Vit D3 . | Control . | ATRA . | Vit D3 . |
| CD14 | 12 | 22 | 100 | 26 | 6 | 100 |
| CD11c | 0.5 | 94 | 98 | 2 | 38 | 78 |
| CD11b | 49 | 100 | 98 | 3 | 96 | 100 |
| NBT | 0 | 36 | 98 | 0 | 1 | 23 |
| . | Mock Clone M6 . | Antisense Rb Clone A1 . | ||||
|---|---|---|---|---|---|---|
| . | Control . | ATRA . | Vit D3 . | Control . | ATRA . | Vit D3 . |
| CD14 | 12 | 22 | 100 | 26 | 6 | 100 |
| CD11c | 0.5 | 94 | 98 | 2 | 38 | 78 |
| CD11b | 49 | 100 | 98 | 3 | 96 | 100 |
| NBT | 0 | 36 | 98 | 0 | 1 | 23 |
Cells were incubated at an initial concentration of 200,000 cells/mL and induced with ATRA at 1 μmol/L or Vit D3 at 0.1 μmol/L. After 4 days, the cells were subjected to analysis of surface antigens as well as NBT reduction test. Values shown are the percentage of cells expressing surface antigens or reducing NBT determined as described in the Materials and Methods. Results are shown from one representative experiment.