Table 3. Antisense RB Oligonucleotides... | American Society of Hematology

Table 3.

Antisense RB Oligonucleotides Suppressed Differentiation But Not G0/G1 Accumulation on Incubation of Wild-Type U-937 Cells With ATRA or Vit D3

Day		No Inducer			Vit D3			ATRA
		Buffer	s-Rb	a-Rb	Buffer	s-Rb	a-Rb	Buffer	s-Rb	a-Rb
0	G0/G1	57	56	64	54	59	60	56	60	58
4	G0/G1	78	78	74	96	97	98	96	95	85
4	NBT	0	0	0	86	83	26	39	42	1

Day		No Inducer			Vit D3			ATRA
		Buffer	s-Rb	a-Rb	Buffer	s-Rb	a-Rb	Buffer	s-Rb	a-Rb
0	G0/G1	57	56	64	54	59	60	56	60	58
4	G0/G1	78	78	74	96	97	98	96	95	85
4	NBT	0	0	0	86	83	26	39	42	1

Cells were preincubated for 24 hours with buffer, sense RB oligos (s-Rb; 50 μmol/L), and antisense RB oligos (a-Rb; 50 μmol/L); respectively. The incubated cells were then induced with ATRA of 1 μmol/L or Vit D3 at 0.1 μmol/L at a concentration of 200,000 cells/mL. After 4 days, cells were again subjected to determination of cell cycle phase distribution by flow cytometric analysis as well as an NBT reduction test. Values shown are the percentages of cells in G0/G1 or reducing NBT. Results are from one representative experiment.

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