Antisense Rb-Transfected Cells Showed Decreased Ability to Differentiate But Not to Accumulate in G0/G1 as Compared With Mock-Transfected Cells on Incubation With ATRA or Vit D3
| Day . | . | Mock Clone M6 . | Antisense Rb Clone A1 . | ||||
|---|---|---|---|---|---|---|---|
| . | . | Control . | ATRA . | Vit D3 . | Control . | ATRA . | Vit D3 . |
| 0 | G0/G1 | 54 | 55 | ||||
| 4 | G0/G1 | 65 | 97 | 97 | 75 | 92 | 99 |
| 4 | NBT | 0 | 29 | 98 | 0 | 0 | 27 |
| Day . | . | Mock Clone M6 . | Antisense Rb Clone A1 . | ||||
|---|---|---|---|---|---|---|---|
| . | . | Control . | ATRA . | Vit D3 . | Control . | ATRA . | Vit D3 . |
| 0 | G0/G1 | 54 | 55 | ||||
| 4 | G0/G1 | 65 | 97 | 97 | 75 | 92 | 99 |
| 4 | NBT | 0 | 29 | 98 | 0 | 0 | 27 |
Cells were incubated at a concentration of 200,000 cells/mL and induced with ATRA at 1 mmol/L or Vit D3 at 0.1 μmol/L. After 4 days, cells were again subjected to determination of cell cycle phase distribution by flow cytometric analysis as well as an NBT reduction test. Values shown are the percentages of cells in G0/G1 or reducing NBT. Results are from one representative experiment.