Rb-Transfected U-937 Cells Accumulated in G0/G1 to a Similar Extent as Mock-Transfected Clones Upon Incubation With ATRA or Vit D3
| Day . | . | Mock Clones (M2; M6) . | Rb Clones (B3; B4) . | ||||
|---|---|---|---|---|---|---|---|
| . | . | Control . | ATRA . | Vit D3 . | Control . | ATRA . | Vit D3 . |
| 4 | G0/G1 | 60; 58 | 94; 96 | 96; 97 | 50; 71 | 90; 97 | 96; 98 |
| Day . | . | Mock Clones (M2; M6) . | Rb Clones (B3; B4) . | ||||
|---|---|---|---|---|---|---|---|
| . | . | Control . | ATRA . | Vit D3 . | Control . | ATRA . | Vit D3 . |
| 4 | G0/G1 | 60; 58 | 94; 96 | 96; 97 | 50; 71 | 90; 97 | 96; 98 |
Cells from two mock-transfected clones (M2; M6) and two RB-transfected clones (B3; B4) were incubated at a concentration of 200,000 cells/mL and induced with ATRA at 1 μmol/L or Vit D3 at 0.1 μmol/L. After 4 days, cells were subjected to determination of cell cycle phase distribution by flow cytometric analysis. Values shown are the percentages of cells of the two mock-transfected and the two RB-transfected clones in G0/G1. Results are from one representative experiment.