Effect of rhIL-4 and rhIL-10 on Cytokine Secretion by PBMC
| . | . | — . | +rhIL-4 (1,000 pg/mL) . | +rhIL-10 (2,500 pg/mL) . | |||
|---|---|---|---|---|---|---|---|
| . | . | p40 (pg/mL) . | IFN-γ (pg/mL) . | p40 (pg/mL) . | IFN-γ (pg/mL) . | p40 (pg/mL) . | IFN-γ (pg/mL) . |
| Exp 1 | LPS | 106 | 404 | 114 | 430 | 15 | 0 |
| Exp 2 | LPS | 132 | 410 | 198 | 422 | 36 | 0 |
| Exp 1 | SAC | 572 | 694 | 618 | 572 | 151 | 378 |
| Exp 2 | SAC | 251 | 868 | 137 | 704 | 45 | 472 |
| . | . | — . | +rhIL-4 (1,000 pg/mL) . | +rhIL-10 (2,500 pg/mL) . | |||
|---|---|---|---|---|---|---|---|
| . | . | p40 (pg/mL) . | IFN-γ (pg/mL) . | p40 (pg/mL) . | IFN-γ (pg/mL) . | p40 (pg/mL) . | IFN-γ (pg/mL) . |
| Exp 1 | LPS | 106 | 404 | 114 | 430 | 15 | 0 |
| Exp 2 | LPS | 132 | 410 | 198 | 422 | 36 | 0 |
| Exp 1 | SAC | 572 | 694 | 618 | 572 | 151 | 378 |
| Exp 2 | SAC | 251 | 868 | 137 | 704 | 45 | 472 |
Whole blood obtained from healthy individuals (n = 2) was preincubated with either rhIL-4 (1000 pg/mL) or rhIL-10 (2500 pg/mL) for 12 hours. Thereafter, PBMC were isolated and stimulated with either LPS (1 ng/mL) or SAC (0.075% wt/vol) for 24 hours. Levels of IL-12 p40 and IFN-γ in supernatants from PBMC were determined with specific ELISA.
Abbreviation: Exp, experiment.