Lymphocyte Adherence to KG1a
| KG1a Treatment* . | Mean (SEM) of Binding (% of untreated control)† . |
|---|---|
| Neuraminidase (t = 0, no culture) | 0.02 (0.1)‡ |
| Neuraminidase buffer control (no culture) | 98.5 (7.5) |
| Neuraminidase, 24-hr culture | 101.3 (6.5) |
| Neuraminidase, 24-hr culture with chlorate (10 mmol/L) | 107.1 (6.2) |
| Neuraminidase, 20-hr culture with tunicamycin (15 μg/mL) | 0.4 (0.1)‡ |
| Neuraminidase, 20-hr culture with tunicamycin buffer alone | 105.2 (7.5) |
| 20-hr culture with tunicamycin (15 μg/mL) (no neuraminidase pretreatment) | 106.2 (7.2) |
| Neuraminidase, 20-hr culture with cycloheximide (1.25 μg/mL) | 0.5 (0.2)‡ |
| 20-hr culture with cycloheximide (1.25 μg/mL) (no neuraminidase pretreatment) | 91.8 (5.8) |
| KG1a Treatment* . | Mean (SEM) of Binding (% of untreated control)† . |
|---|---|
| Neuraminidase (t = 0, no culture) | 0.02 (0.1)‡ |
| Neuraminidase buffer control (no culture) | 98.5 (7.5) |
| Neuraminidase, 24-hr culture | 101.3 (6.5) |
| Neuraminidase, 24-hr culture with chlorate (10 mmol/L) | 107.1 (6.2) |
| Neuraminidase, 20-hr culture with tunicamycin (15 μg/mL) | 0.4 (0.1)‡ |
| Neuraminidase, 20-hr culture with tunicamycin buffer alone | 105.2 (7.5) |
| 20-hr culture with tunicamycin (15 μg/mL) (no neuraminidase pretreatment) | 106.2 (7.2) |
| Neuraminidase, 20-hr culture with cycloheximide (1.25 μg/mL) | 0.5 (0.2)‡ |
| 20-hr culture with cycloheximide (1.25 μg/mL) (no neuraminidase pretreatment) | 91.8 (5.8) |
After neuraminidase treatment, all cells were washed before culturing as indicated. Experimental details are described in the text.
Number of lymphocytes adherent to confluent area of KG1a were counted by light microscopy using an ocular grid under 250× magnification (quantified a minimum of 2 fields/slide, 2 slides/experiment, 3 separate experiments). Results are presented as the percentage of binding compared with corresponding untreated control KG1a cytospin preparations.
Statistically significant ( P < .0001, by paired t-test) difference compared with neuraminidase buffer control cells. All other values are not statistically different (P > .05).