CD19 Modulation with Anti-B4 and Anti-B4–bR in Namalwa Cells
| Treatment . | MFI . | Modulation (%) . | |
|---|---|---|---|
| . | O°C . | 37°C . | . |
| N901-bR | 4 | 4 | — |
| Anti-B4 | 138 | 119 | 14 |
| Anti-B4–bR | 142 | 93 | 36 |
| Anti-B4–bR + lactose (50 mmol/L) | 141 | 119 | 16 |
| Anti-B4–bR + lactose (100 mmol/L) | 145 | 130 | 11 |
| Treatment . | MFI . | Modulation (%) . | |
|---|---|---|---|
| . | O°C . | 37°C . | . |
| N901-bR | 4 | 4 | — |
| Anti-B4 | 138 | 119 | 14 |
| Anti-B4–bR | 142 | 93 | 36 |
| Anti-B4–bR + lactose (50 mmol/L) | 141 | 119 | 16 |
| Anti-B4–bR + lactose (100 mmol/L) | 145 | 130 | 11 |
Cells were mixed with anti-B4, anti-B4–bR, or anti-B4–bR plus lactose at 0°C and incubated for 2.5 hours at 37°C in the continuous presence of the reagents. Control cultures were incubated with N901-bR. After washings with cold medium, cells were stained with GAM-FITC to detect MoAb or IT remaining on the cell surface.