Binding and Uptake of Radiolabeled Anti-B4 and Anti-B4–bR by Namalwa Cells
| Material . | Molecules/Cell . | Ratio of Molecules Inside/Molecules Outside . | ||
|---|---|---|---|---|
| . | Total Cell-Associated Molecules . | Internalized Molecules . | Surface-Bound Molecules4-150 . | . |
| Anti-B4–bR | 2.1 ± 0.3 × 104 | 5.8 ± 0.4 × 103 | 1.5 ± 0.3 × 104 | 0.39 ± 0.09 |
| Anti-B4 | 1.8 ± 0.3 × 104 | 1.8 ± 0.3 × 103 | 1.6 ± 0.3 × 104 | 0.11 ± 0.03 |
| Material . | Molecules/Cell . | Ratio of Molecules Inside/Molecules Outside . | ||
|---|---|---|---|---|
| . | Total Cell-Associated Molecules . | Internalized Molecules . | Surface-Bound Molecules4-150 . | . |
| Anti-B4–bR | 2.1 ± 0.3 × 104 | 5.8 ± 0.4 × 103 | 1.5 ± 0.3 × 104 | 0.39 ± 0.09 |
| Anti-B4 | 1.8 ± 0.3 × 104 | 1.8 ± 0.3 × 103 | 1.6 ± 0.3 × 104 | 0.11 ± 0.03 |
Values are the mean ± SEM of two independent experiments, each performed in duplicate.
Cell surface-bound molecules are derived from total number of cell-associated molecules minus the number of internalized molecules.