Cell Cycle Status of Lin− Rholow/Holow-Derived HPP-CFC Cultured in Cytokines
| Time in Culture . | HPP-CFC/100 Cells* . | % Killed by 3H-TdR† . |
|---|---|---|
| 0 h | 28 ± 13 | 3 ± 3 |
| 28-30 h | 31 ± 7 | 60 ± 10‡ |
| Time in Culture . | HPP-CFC/100 Cells* . | % Killed by 3H-TdR† . |
|---|---|---|
| 0 h | 28 ± 13 | 3 ± 3 |
| 28-30 h | 31 ± 7 | 60 ± 10‡ |
Seven-factor–responsive HPP-CFC per 100 cells from initially isolated Lin− Rholow Holow cells and after 28 to 30 hours in culture with IL-3, IL-11, IL-6, and SCF (see the Materials and Methods).
Cell cycle status of HPP-CFC as determined by 3H-TdR suicide. These data are from four separate experiments with 4 to 10 culture plates per group in each experiment.
Significantly different from 0 hours value (Wilcoxon rank-sum test; P = .0209).