Effect of MoAbs Blocking vWF Binding to Platelet GPIbα
| MoAb . | Adherent Cells (% of total) . | ||
|---|---|---|---|
| Target . | Clone . | WT-rvWF . | RGGS-rvWF . |
| None | — | 45.4 ± 2.4 (n = 4) | 16.6 ± 1.1 (n = 4) |
| vWF | 723 | 45.2 ± 1.9 (n = 4) | 17.3 ± 1 (n = 4) |
| vWF | 713 | 43.7 ± 1.7 (n = 4) | 17.9 ± 1.3 (n = 4) |
| vWF | 724 | 39.6 ± 4.6 (n = 4) | 17.1 ± 1.3 (n = 3) |
| GPIbα | 6D1 | 42.6 ± 3.9 (n = 4) | 16.8 ± 1.2 (n = 3) |
| GPIbα | AS-7 | 39.7 ± 7.1 (n = 4) | 15.6 ± 1 (n = 3) |
| GPIbα | Ib-23 | 46.6 (n = 2) | 17.7 (n = 2) |
| GPIbα | SZ2 | 43.7 (n = 2) | 19.1 (n = 2) |
| GPIX | SZ1 | 45.5 (n = 2) | 16.6 (n = 2) |
| MoAb . | Adherent Cells (% of total) . | ||
|---|---|---|---|
| Target . | Clone . | WT-rvWF . | RGGS-rvWF . |
| None | — | 45.4 ± 2.4 (n = 4) | 16.6 ± 1.1 (n = 4) |
| vWF | 723 | 45.2 ± 1.9 (n = 4) | 17.3 ± 1 (n = 4) |
| vWF | 713 | 43.7 ± 1.7 (n = 4) | 17.9 ± 1.3 (n = 4) |
| vWF | 724 | 39.6 ± 4.6 (n = 4) | 17.1 ± 1.3 (n = 3) |
| GPIbα | 6D1 | 42.6 ± 3.9 (n = 4) | 16.8 ± 1.2 (n = 3) |
| GPIbα | AS-7 | 39.7 ± 7.1 (n = 4) | 15.6 ± 1 (n = 3) |
| GPIbα | Ib-23 | 46.6 (n = 2) | 17.7 (n = 2) |
| GPIbα | SZ2 | 43.7 (n = 2) | 19.1 (n = 2) |
| GPIX | SZ1 | 45.5 (n = 2) | 16.6 (n = 2) |
Nonstimulated endothelial cells were preincubated for 30 minutes at 4°C with 20 μg/mL of purified IgG corresponding to the different clones indicated in the table. The percentage of cell adhesion was measured as described in the Materials and Methods. The number of experiments in duplicate is indicated into parenthesis. None of the anti-vWF or anti-GPIbα antibodies had any effect on endothelial cell adhesion to WT-rvWF or RGGS-rvWF.