Table 3. Supernatants From T-Cell... | American Society of Hematology

Table 3.

Supernatants From T-Cell Cultures From Patients With ALD and From Normal Donors Do Not Affect Fas-Mediated Cell Death in T-Cell Cultures

Cells^3-151	Medium	Supernatant^3-150
		Patient 1	Patient 5	Patient 6	Control 1	Control 2	Control 3
Experiment 1
Patient 1	99^c	100	99	100	99	99	100
Control 1	67	61	61	62	66	62	62
Control 2	56	52	56	56	50	53	55
Control 3	55	52	50	53	50	55	52
Experiment 2	Medium	Patient 3	Control 4	Control 5	Control 6
Patient 3	100	100	91	90	98
Control 4	60	57	60	55	54
Control 5	26	33	32	35	30
Control 6	56	51	51	57	54
Experiment 3	Medium	Patient 2	Control 7
Patient 2	100	91	95
Control 7	52	53	58

Cells^3-151	Medium	Supernatant^3-150
		Patient 1	Patient 5	Patient 6	Control 1	Control 2	Control 3
Experiment 1
Patient 1	99^c	100	99	100	99	99	100
Control 1	67	61	61	62	66	62	62
Control 2	56	52	56	56	50	53	55
Control 3	55	52	50	53	50	55	52
Experiment 2	Medium	Patient 3	Control 4	Control 5	Control 6
Patient 3	100	100	91	90	98
Control 4	60	57	60	55	54
Control 5	26	33	32	35	30
Control 6	56	51	51	57	54
Experiment 3	Medium	Patient 2	Control 7
Patient 2	100	91	95
Control 7	52	53	58

F3-150

Supernatants from T-cell culture obtained from patients with ALD and age-matched normal donors (controls) were added to the Fas-mediated cell death assay at a final dilution of 1:4.

F3-151

Cells used as targets in the Fas-mediated cell death assay. They were PHA-derived T-cell lines obtained from patients with ALD and normal controls as described in Materials and Methods.

F3-152

Cells were incubated for 18 hours with control medium or anti-Fas MoAb (1 μg/mL). Then total cell survival was assessed by the trypan blue exclusion test. Results are expressed as percentage of cell survival.

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