Table 1.

Retroviral Transduction of CD34+ Cell Subsets: Effect of FLT3L

Cells4 HGF4 HGF + FLT3L
CD34+CD38+ 20 (39/192) 19 (33/173) 
CD34+CD38+/− 15 (34/226) 22 (43/196) 
CD34+CD38 7.5 (12/159) 15 (28/182) 
Cells4 HGF4 HGF + FLT3L
CD34+CD38+ 20 (39/192) 19 (33/173) 
CD34+CD38+/− 15 (34/226) 22 (43/196) 
CD34+CD38 7.5 (12/159) 15 (28/182) 

CD34+CD38+, CD34+CD38+/−, and CD34+CD38 cells were cultured with LAPSN retrovirus with either 4 HGF or 4 HGF + FLT3L. Cells were harvested after 2.5 days in the presence of retrovirus and total of 10 days culture in HGF. Cytocentrifuge preparations were stained for akaline phosphatase and the number of positive cells were expressed as a proportion of total cells. The figures in the table represent the percentage of alkaline phosphatase positive cells (number of positive/total cells examined).

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