Class Analysis of Murine CD34 Using Human CD34 Epitope Subclass Criteria29 Indicates That RAM34 Recognizes a Class III Epitope
| . | Class I . | . | Class II . | . | Class III . | |||
|---|---|---|---|---|---|---|---|---|
| . | Neuraminidase Sensitive . | . | . | Glycoprotease Sensitive/Neuraminidase Resistant . | . | . | Glycoprotease Resistant/Neuraminidase Resistant . | . |
| Cell Line | Human CD34 (IMMU 133) | Mouse CD34 (RAM34) | Human CD34 (QBEND 10) | Mouse CD34 (RAM34) | Human CD34 (HPCA2) | Mouse CD34 (RAM34) | ||
| KG1A (human progenitor cell line) | + | N/A | + | N/A | + | N/A | ||
| 416B (mouse progenitor cell line) | N/A | − | N/A | − | N/A | + | ||
| 416B/HU CD34 (mouse line transfected with human CD34) | + | − | + | − | + | + | ||
| . | Class I . | . | Class II . | . | Class III . | |||
|---|---|---|---|---|---|---|---|---|
| . | Neuraminidase Sensitive . | . | . | Glycoprotease Sensitive/Neuraminidase Resistant . | . | . | Glycoprotease Resistant/Neuraminidase Resistant . | . |
| Cell Line | Human CD34 (IMMU 133) | Mouse CD34 (RAM34) | Human CD34 (QBEND 10) | Mouse CD34 (RAM34) | Human CD34 (HPCA2) | Mouse CD34 (RAM34) | ||
| KG1A (human progenitor cell line) | + | N/A | + | N/A | + | N/A | ||
| 416B (mouse progenitor cell line) | N/A | − | N/A | − | N/A | + | ||
| 416B/HU CD34 (mouse line transfected with human CD34) | + | − | + | − | + | + | ||