Adhesion and Invasion of c-MET Positive and Negative B-Cell Lines to Rat Fibroblast Monolayers
| Cells . | HGF (ng/mL) . | Adhesion (%)* . | Invasion (%)*† . |
|---|---|---|---|
| BJAB | 0 | 1.8 | 2.2 |
| 5 | 4.4 | 2.2 | |
| 15 | 7.2 | 3.8 | |
| Raji | 0 | 2.0 | 11.6 |
| 5 | 1.4 | 12.4 | |
| 15 | 2.2 | 24.6 | |
| Daudi | 0 | 1.0 | 0.8 |
| 5 | 0.8 | 2.2 | |
| 15 | 1.0 | 1.4 |
| Cells . | HGF (ng/mL) . | Adhesion (%)* . | Invasion (%)*† . |
|---|---|---|---|
| BJAB | 0 | 1.8 | 2.2 |
| 5 | 4.4 | 2.2 | |
| 15 | 7.2 | 3.8 | |
| Raji | 0 | 2.0 | 11.6 |
| 5 | 1.4 | 12.4 | |
| 15 | 2.2 | 24.6 | |
| Daudi | 0 | 1.0 | 0.8 |
| 5 | 0.8 | 2.2 | |
| 15 | 1.0 | 1.4 |
Percent of invading and adhering cells from added cells calculated from 20 “fields” counted through an ocular of inverted microscope from a representative experiment (N = 3).
Fibroblasts that were dividing looked like invaded cells, because they were round instead of elongated, resulting in a threshold of invading cells of ± 2%.