Summary of Blood Cell Repopulating Profiles Observed in the Competitive Reconstitution Assay
| KTLS/KTLS Subset . | No. of Cells Injected . | Total No. of Mice . | No. of Mice . | |||
|---|---|---|---|---|---|---|
| . | . | . | LTMR . | STMR . | B + T . | Single Lineage . |
| From HU-treated BM | ||||||
| KTLS | 10 | 23 | 7 | 10 | 2 | 2 |
| 50 | 25 | 18 | 7 | 0 | 0 | |
| 100 | 17 | 16 | 1 | 0 | 0 | |
| G0/G1 Rh123lo | 0.83 | 24 | 0 | 3 | 0 | 2 |
| 5 | 15 | 2 | 4 | 2 | 0 | |
| 10 | 27 | 8 | 11 | 1 | 4 | |
| 20 | 4 | 4 | 0 | 0 | 0 | |
| 50 | 8 | 8 | 0 | 0 | 0 | |
| 100 | 5 | 5 | 0 | 0 | 0 | |
| S/G2/M Rh123med | 10 | 20 | 3 | 8 | 2 | 3 |
| 50 | 6 | 3 | 3 | 0 | 0 | |
| From normal mouse BM | ||||||
| G0/G1 Rh123lo | 0.83 | 19 | 1 | 0 | 0 | 1 |
| 5 | 14 | 1 | 4 | 1 | 2 | |
| 10 | 8 | 2 | 2 | 1 | 0 | |
| 20 | 3 | 3 | 0 | 0 | 0 | |
| KTLS/KTLS Subset . | No. of Cells Injected . | Total No. of Mice . | No. of Mice . | |||
|---|---|---|---|---|---|---|
| . | . | . | LTMR . | STMR . | B + T . | Single Lineage . |
| From HU-treated BM | ||||||
| KTLS | 10 | 23 | 7 | 10 | 2 | 2 |
| 50 | 25 | 18 | 7 | 0 | 0 | |
| 100 | 17 | 16 | 1 | 0 | 0 | |
| G0/G1 Rh123lo | 0.83 | 24 | 0 | 3 | 0 | 2 |
| 5 | 15 | 2 | 4 | 2 | 0 | |
| 10 | 27 | 8 | 11 | 1 | 4 | |
| 20 | 4 | 4 | 0 | 0 | 0 | |
| 50 | 8 | 8 | 0 | 0 | 0 | |
| 100 | 5 | 5 | 0 | 0 | 0 | |
| S/G2/M Rh123med | 10 | 20 | 3 | 8 | 2 | 3 |
| 50 | 6 | 3 | 3 | 0 | 0 | |
| From normal mouse BM | ||||||
| G0/G1 Rh123lo | 0.83 | 19 | 1 | 0 | 0 | 1 |
| 5 | 14 | 1 | 4 | 1 | 2 | |
| 10 | 8 | 2 | 2 | 1 | 0 | |
| 20 | 3 | 3 | 0 | 0 | 0 | |
Lethally irradiated host mice were competitively reconstituted with Ly-5–marked KTLS or KTLS subsets along with 105 host-type BM cells. The type of reconstitution was categorized as LTMR, STMR, B + T, or single lineage based on detection of Ly-5–marked, donor-derived blood cells in the BM of the reconstituted mouse.
Abbreviations: LTMR, detection of long-term (≥6 months) donor-derived myeloid, B cells, and T cells (except in 2 mice that did not produce donor-derived T cells); STMR, detection of transient donor-derived myeloid cells plus B and/or T cells during screening period; B + T, detection of donor-derived B and T cells; single lineage, detection of a single donor-derived lineage (ie, myeloid, B, or T cells; most animals were reconstituted with donor-derived B cells).