Engraftment Frequency of KTLS, G0/G1 Rh123lo KTLS, and S/G2/M Rh123mid KTLS Cells From HU-Treated Mice
| KTLS Subset . | Engraftment Frequency . | LTMR Only ≥6 Mo . | No. of Mice Screened . | ||
|---|---|---|---|---|---|
| . | of Injected Cells . | (1/frequency) . | CI . | . | |
| . | (1/frequency) . | CI . | . | . | . |
| From HU-treated BM | |||||
| KTLS | 4.9 | 3.2-10.1 | 35.6 | 26.3-54.9 | 65 |
| G0/G1 Rh123lo | 4.9 | 3.6-7.5 | 21.6 | 15.0-38.5 | 83 |
| S/G2/M Rh123med | 6.2 | 4.0-13.6 | 66.7 | 36.9-350.8 | 26 |
| From normal mouse BM | |||||
| G0/G1 Rh123lo | 7.2 | 4.8-14.3 | 25.5 | 14.6-102.1 | 44 |
| KTLS Subset . | Engraftment Frequency . | LTMR Only ≥6 Mo . | No. of Mice Screened . | ||
|---|---|---|---|---|---|
| . | of Injected Cells . | (1/frequency) . | CI . | . | |
| . | (1/frequency) . | CI . | . | . | . |
| From HU-treated BM | |||||
| KTLS | 4.9 | 3.2-10.1 | 35.6 | 26.3-54.9 | 65 |
| G0/G1 Rh123lo | 4.9 | 3.6-7.5 | 21.6 | 15.0-38.5 | 83 |
| S/G2/M Rh123med | 6.2 | 4.0-13.6 | 66.7 | 36.9-350.8 | 26 |
| From normal mouse BM | |||||
| G0/G1 Rh123lo | 7.2 | 4.8-14.3 | 25.5 | 14.6-102.1 | 44 |
The frequency of donor KTLS cells and subsets contributing to blood cell production in the competitive reconstitution assay was calculated based on linear regression analysis. Engraftment frequency includes all repopulation types, ie, LTMR, STMR, B + T cells, and single lineage as summarized in Table 2.
Abbreviation: CI, 95% confidence interval.