Effect of Various Agonists Toward Nuclear Mobilization of NF-κB/Rel Proteins in Human Neutrophils
| Agonist . | Stimulation Time (min) . | ||
|---|---|---|---|
| . | 10 . | 60 . | 120 . |
| fMLP (10 nmol/L) | − | + | + |
| G-CSF (1,000 U/mL) | − | − | − |
| GM-CSF (1 nmol/L) | − | − | − |
| IL-1β (10 ng/mL) | ± | ± | ± |
| IL-8 (10 nmol/L) | − | − | − |
| IL-10 (100 U/mL) | − | − | − |
| IFN-γ (100 U/mL) | − | − | − |
| LPS (1 μg/mL) | ++ | ++ | ++ |
| LTB4 (10 nmol/L) | − | ± | ± |
| PAF (100 nmol/L) | − | ± | ± |
| PMA (20 ng/mL) | − | + | + |
| TNFα (100 U/mL) | ++ | ++ | ++ |
| Agonist . | Stimulation Time (min) . | ||
|---|---|---|---|
| . | 10 . | 60 . | 120 . |
| fMLP (10 nmol/L) | − | + | + |
| G-CSF (1,000 U/mL) | − | − | − |
| GM-CSF (1 nmol/L) | − | − | − |
| IL-1β (10 ng/mL) | ± | ± | ± |
| IL-8 (10 nmol/L) | − | − | − |
| IL-10 (100 U/mL) | − | − | − |
| IFN-γ (100 U/mL) | − | − | − |
| LPS (1 μg/mL) | ++ | ++ | ++ |
| LTB4 (10 nmol/L) | − | ± | ± |
| PAF (100 nmol/L) | − | ± | ± |
| PMA (20 ng/mL) | − | + | + |
| TNFα (100 U/mL) | ++ | ++ | ++ |
Cells (5 × 106 mL) were cultured at 37°C in the presence or absence of the agonists listed below; aliquots were taken at the indicated times and submitted to nonionic detergent lysis. The resulting nuclear fractions were then processed for electrophoresis and immunoblotting using antisera to p50, p65/RelA, or c-Rel, as described in Materials and Methods. The data summarized in this table are representative of at least three independent experiments. For purposes of clarity, only the relative magnitude of the effect of the agonists is indicated. −, no detectable effect; ±, weak but significant effect; + or ++, strong effect. As an approximate reference, a weak effect corresponds to a difference such as the one observed between the 40-minute and 60-minute time points in Fig 3 (c-Rel, fMLP-stimulated cells).