Effect of Various Agonists Toward NF-κB Activation in Human Neutrophils
| Agonist . | Stimulation Time (min) . | ||
|---|---|---|---|
| . | 10 . | 40 . | 60 . |
| fMLP (10 nmol/L) | − | + | + |
| G-CSF (1,000 U/mL) | − | − | − |
| GM-CSF (1 nmol/L) | − | − | − |
| IL-1β (10 ng/mL) | + | + | − |
| IL-8 (10 nmol/L) | − | − | − |
| IL-10 (100 U/mL) | − | − | − |
| IFNα (1,000 U/mL) | − | − | − |
| IFN-γ (100 U/mL) | − | − | − |
| LPS (1 μg/mL) | ++ | ++ | + |
| LTB4 (10 nmol/L) | − | + | + |
| PAF (100 nmol/L) | − | + | + |
| PMA (20 ng/mL) | − | + | + |
| TNFα (100 U/mL) | ++ | ++ | + |
| Agonist . | Stimulation Time (min) . | ||
|---|---|---|---|
| . | 10 . | 40 . | 60 . |
| fMLP (10 nmol/L) | − | + | + |
| G-CSF (1,000 U/mL) | − | − | − |
| GM-CSF (1 nmol/L) | − | − | − |
| IL-1β (10 ng/mL) | + | + | − |
| IL-8 (10 nmol/L) | − | − | − |
| IL-10 (100 U/mL) | − | − | − |
| IFNα (1,000 U/mL) | − | − | − |
| IFN-γ (100 U/mL) | − | − | − |
| LPS (1 μg/mL) | ++ | ++ | + |
| LTB4 (10 nmol/L) | − | + | + |
| PAF (100 nmol/L) | − | + | + |
| PMA (20 ng/mL) | − | + | + |
| TNFα (100 U/mL) | ++ | ++ | + |
Cells (5 × 106/mL) were cultured at 37°C in the presence or absence of the agonists listed below; aliquots were taken at the indicated times, and cells were disrupted by nitrogen cavitation. The resulting nuclear fractions were then processed for EMSA using an oligonucleotide probe containing a consensus NF-κB sequence, as described in Materials and Methods. The data summarized in this table are representative of at least three independent experiments. For purposes of clarity, only the relative magnitude of the effect of the agonists is indicated: −, little or no detectable effect; +, weak but significant effect; ++, strong effect. The data presented in Fig 7B can serve as an approximate reference of the relative ability of some of these stimuli to induce NF-κB activation.