Effect of Various Cytokines on the Percentage of Primary αIIbβ3+ BM Cells Undergoing Apoptosis in Liquid Cultures
| Cytokines . | Days of Culture . | |||
|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 6 . |
| None | 9 ± 2.7 | 22 ± 4.5 | 36 ± 6 | 52 ± 7 |
| TPO (100 ng/mL) | 9 ± 2.7 | 11.5 ± 2 | 19 ± 4 | 46 ± 6 |
| EPO (10 ng/mL) | 9 ± 2.7 | 20 ± 4.8 | 31 ± 5 | 56 ± 8 |
| IL-3 (10 ng/mL) | 9 ± 2.7 | 16 ± 5 | 29 ± 7 | 49 ± 8 |
| TPO (100 ng/mL) + IL-3 (10 ng/mL) | 9 ± 2.7 | 10 ± 3 | 17 ± 5 | 43 ± 7.7 |
| Cytokines . | Days of Culture . | |||
|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 6 . |
| None | 9 ± 2.7 | 22 ± 4.5 | 36 ± 6 | 52 ± 7 |
| TPO (100 ng/mL) | 9 ± 2.7 | 11.5 ± 2 | 19 ± 4 | 46 ± 6 |
| EPO (10 ng/mL) | 9 ± 2.7 | 20 ± 4.8 | 31 ± 5 | 56 ± 8 |
| IL-3 (10 ng/mL) | 9 ± 2.7 | 16 ± 5 | 29 ± 7 | 49 ± 8 |
| TPO (100 ng/mL) + IL-3 (10 ng/mL) | 9 ± 2.7 | 10 ± 3 | 17 ± 5 | 43 ± 7.7 |
Apoptosis was evaluated by the TUNEL technique and shown by flow cytometry. Data are expressed as the means ± SD of four to seven separate experiments, each performed in duplicate starting from a different BM sample.