IgE-Mediated Release of MCP-1 From Isolated Lung MC
| Challenge . | MCP-1, pg per mL supernatant (106 cells per mL) . | |||
|---|---|---|---|---|
| . | Donor 1 (%) . | Donor 2 (%) . | Donor 3 (%) . | Mean ± SD (%) . |
| Control buffer | 155.8 (100) | 162.8 (100) | 288.3 (100) | 202.3 ± 21.0 (100) |
| Anti-IgE, 10 μg/mL* | 358.8 (230) | 771.5 (474) | 529.5 (184) | 553.3 ± 210.6* (274) |
| Anti-IgE, 1 μg/mL | 210.8 (135) | 375.1 (230) | 348.5 (121) | 311.5 ± 116.2 (154) |
| Anti-IgE, 0.1 μg/mL | 140.2 (90) | 212.6 (131) | 280.0 (97) | 210.9 ± 34.0 (104) |
| Challenge . | MCP-1, pg per mL supernatant (106 cells per mL) . | |||
|---|---|---|---|---|
| . | Donor 1 (%) . | Donor 2 (%) . | Donor 3 (%) . | Mean ± SD (%) . |
| Control buffer | 155.8 (100) | 162.8 (100) | 288.3 (100) | 202.3 ± 21.0 (100) |
| Anti-IgE, 10 μg/mL* | 358.8 (230) | 771.5 (474) | 529.5 (184) | 553.3 ± 210.6* (274) |
| Anti-IgE, 1 μg/mL | 210.8 (135) | 375.1 (230) | 348.5 (121) | 311.5 ± 116.2 (154) |
| Anti-IgE, 0.1 μg/mL | 140.2 (90) | 212.6 (131) | 280.0 (97) | 210.9 ± 34.0 (104) |
Human lung MC (three donors) were isolated as described in the text and kept in culture (RPMI 1640 medium plus 10% FCS) for 24 hours before release experiments were performed. MC were first incubated with IgE (10 μg/mL, 3 hours). Cells were then washed and exposed to control medium or anti-IgE MoAb E-124-2-8 (concentrations as indicated) at 37°C for 45 minutes. Thereafter, cells were centrifuged and the cell-free supernatants recovered and analyzed for the presence of MCP-1 by ELISA. The results show the MCP-1 concentrations (pg per mL) in the supernatants (mean of duplicates) in the three donors, and the mean ± SD of all data in the three donors.
A significant increase in MCP-1 in the supernatants as compared with control (P < .05) was obtained using 10 μg anti-IgE per mL.