Dose-Dependent Expression and Release of MCP-1 in HMC-1 Cells in Response to SCF
| Stimulus . | MCP-1 Concentration, % of Control . | |||
|---|---|---|---|---|
| . | Sup 8 h . | Lysates 8 h . | Sup 12 h . | Lysates 12 h . |
| none | 100 ± 8 | 100 ± 23 | 100 ± 4 | 100 ± 25 |
| SCF, 1 ng/mL | 200 ± 18 | 258 ± 85 | 282 ± 22 | 235 ± 14 |
| SCF, 5 ng/mL | 215 ± 13 | 294 ± 35 | 295 ± 14 | 361 ± 20 |
| SCF, 10 ng/mL | 228 ± 12 | 483 ± 39 | 305 ± 27 | 324 ± 31 |
| SCF, 50 ng/mL | 458 ± 22 | 643 ± 52 | 480 ± 8 | 620 ± 63 |
| SCF, 100 ng/mL | 409 ± 37 | 611 ± 20 | 443 ± 13 | 475 ± 24 |
| SCF, 200 ng/mL | 466 ± 22 | 506 ± 38 | 442 ± 41 | 353 ± 21 |
| Stimulus . | MCP-1 Concentration, % of Control . | |||
|---|---|---|---|---|
| . | Sup 8 h . | Lysates 8 h . | Sup 12 h . | Lysates 12 h . |
| none | 100 ± 8 | 100 ± 23 | 100 ± 4 | 100 ± 25 |
| SCF, 1 ng/mL | 200 ± 18 | 258 ± 85 | 282 ± 22 | 235 ± 14 |
| SCF, 5 ng/mL | 215 ± 13 | 294 ± 35 | 295 ± 14 | 361 ± 20 |
| SCF, 10 ng/mL | 228 ± 12 | 483 ± 39 | 305 ± 27 | 324 ± 31 |
| SCF, 50 ng/mL | 458 ± 22 | 643 ± 52 | 480 ± 8 | 620 ± 63 |
| SCF, 100 ng/mL | 409 ± 37 | 611 ± 20 | 443 ± 13 | 475 ± 24 |
| SCF, 200 ng/mL | 466 ± 22 | 506 ± 38 | 442 ± 41 | 353 ± 21 |
HMC-1 cells were exposed to various concentrations of rhSCF (as indicated) and PMA (50 ng/mL) for 8 h or 12 h at 37°C in complete medium. After incubation, cells were centrifuged and the supernatants (sup) recovered. MCP-1 was measured in lysates and sups by ELISA. As visible, SCF induced an increase in cellular MCP-1 and MCP-1 release in a dose-dependent manner. The results represent the mean ± SD of triplicate cultures from one experiment. A similar dose-dependency was obtained with SCF in the absence of PMA (not shown).
Abbreviations: sup, supernatants; h, hour.