Measurement of MCP-1 in Various Human Cells by ELISA
| Cell Type . | MCP-1 (pg/mL) . | |
|---|---|---|
| . | 2-Hour Supernatant4-150 . | Cell Lysate4-151 . |
| Lung MC (98% pure) | 576 ± 47 | 326 ± 63 |
| Lung cells without MC (<1%) | 1,227 ± 570 | 170 ± 70 |
| HMC-1 (mast cell line) | 3,483 ± 735 | 2,081 ± 313 |
| PB MNC | 166 ± 120 | 187 ± 35 |
| Blood monocytes (Mo) | 163 ± 20 | 384 ± 128 |
| Eosinophils (HES) | <5 | <5 |
| BM MNC | <5 | <5 |
| HUVEC | >5,000 | NT |
| HL-60 (myeloid cell line) | NT | 350 ± 70 |
| KG-1 (myeloid cell line) | 66 ± 9 | 22 ± 12 |
| U937 (monoblastoid cell line) | <5 | 24 ± 5 |
| Cell Type . | MCP-1 (pg/mL) . | |
|---|---|---|
| . | 2-Hour Supernatant4-150 . | Cell Lysate4-151 . |
| Lung MC (98% pure) | 576 ± 47 | 326 ± 63 |
| Lung cells without MC (<1%) | 1,227 ± 570 | 170 ± 70 |
| HMC-1 (mast cell line) | 3,483 ± 735 | 2,081 ± 313 |
| PB MNC | 166 ± 120 | 187 ± 35 |
| Blood monocytes (Mo) | 163 ± 20 | 384 ± 128 |
| Eosinophils (HES) | <5 | <5 |
| BM MNC | <5 | <5 |
| HUVEC | >5,000 | NT |
| HL-60 (myeloid cell line) | NT | 350 ± 70 |
| KG-1 (myeloid cell line) | 66 ± 9 | 22 ± 12 |
| U937 (monoblastoid cell line) | <5 | 24 ± 5 |
Abbreviations: NT, not tested; PB, peripheral blood; BM, bone marrow; HUVEC, cultured human umbilical vein endothelial cells. MCP-1 was measured by a commercial ELISA.
Supernatants were obtained from cell cultures containing 1 × 106 cells per mL, after a 2-hour incubation period.
Cell lysates were prepared from 105 to 107 cells and results expressed as pg per 106 cells. All results represent the mean ± SD of at least three independent experiments (cell lines) or donors (primary cells).