Checkerboard Analysis of MCP-1 and HMC-1 sup-Induced Migration of Blood Mo
| Lower Chamber . | Upper Chamber, MCP-1, ng/mL . | |||
|---|---|---|---|---|
| MCP-1, ng/mL . | 0 . | 1 . | 5 . | 10 . |
| 0 | 3,217 | 2,841 | 2,567 | 2,234 |
| 1 | 9,828 | 7,968 | 6,211 | 4,209 |
| 5 | 12,606 | 9,752 | 7,687 | 5,097 |
| 10 | 17,388 | 14,590 | 9,683 | 6,679 |
| Lower Chamber | Upper Chamber, % HMC-1 sup | |||
| % HMC-1 sup | 0 | 25 | 50 | 100 |
| 0 | 3,560 | 3,140 | 2,653 | 2,154 |
| 25 | 13,612 | 11,456 | 9,743 | 6,051 |
| 50 | 14,508 | 11,946 | 10,216 | 6,348 |
| 100 | 16,840 | 12,015 | 10,519 | 7,506 |
| Lower Chamber . | Upper Chamber, MCP-1, ng/mL . | |||
|---|---|---|---|---|
| MCP-1, ng/mL . | 0 . | 1 . | 5 . | 10 . |
| 0 | 3,217 | 2,841 | 2,567 | 2,234 |
| 1 | 9,828 | 7,968 | 6,211 | 4,209 |
| 5 | 12,606 | 9,752 | 7,687 | 5,097 |
| 10 | 17,388 | 14,590 | 9,683 | 6,679 |
| Lower Chamber | Upper Chamber, % HMC-1 sup | |||
| % HMC-1 sup | 0 | 25 | 50 | 100 |
| 0 | 3,560 | 3,140 | 2,653 | 2,154 |
| 25 | 13,612 | 11,456 | 9,743 | 6,051 |
| 50 | 14,508 | 11,946 | 10,216 | 6,348 |
| 100 | 16,840 | 12,015 | 10,519 | 7,506 |
Checkerboard analysis of MCP-1–induced and HMC-1 sup-induced migration of blood Mo was done as described in the text. Cells were loaded in the upper chamber and after induction by rhMCP-1 or HMC-1 sup and the numbers of migrated Mo were counted in the lower chambers by dye (calcein AM) staining. Various concentrations of rhMCP-1 or HMC-1 sup were applied in either the lower or upper chambers of the chemotaxis assay system, or into both (upper and lower chambers). Selective migration of Mo against MCP-1 and HMC-1-sup suggests a chemotactic response.