Colony Formation by PB-Derived CD34+IL-6R− Cells in Serum-Free Culture
| Factors . | Colony Types . | % of . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | G . | M . | GM . | B . | Eo . | E-Mix . | Total . | Control . |
| None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 |
| SCF | 1 ± 1 | 0 | 0 | 0 | 0 | 0 | 1 ± 1 | 1.0 |
| IL-3 | 0 | 0 | 2 ± 1 | 0 | 8 ± 1 | 0 | 9 ± 0 | 8.7 |
| IL-6 + sIL-6R | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 |
| SCF + IL-3 | 4 ± 2 (NS) | 0 | 5 ± 2 | 0 | 5 ± 1 | 0 | 14 ± 3 | 13.6 |
| SCF + IL-6 + sIL-6R | 2 ± 1 (NS) | 0 | 2 ± 0 | 1 ± 0 (NS) | 0 | 2 ± 1 (NS) | 6 ± 0 | 5.8 |
| IL-3 + IL-6 + sIL-6R | 0 | 0 | 4 ± 1 | 2 ± 0 (NS) | 6 ± 1 | 1 ± 0 (NS) | 12 ± 0 | 11.7 |
| SCF + IL-3 + IL-6 + sIL-6R | 8 ± 1* | 0 | 5 ± 1 | 52 ± 3† | 5 ± 2 | 19 ± 1† | 88 ± 3 | 85.4 |
| CSFs | 11 ± 1 | 1 ± 1 | 18 ± 7 | 55 ± 6 | 2 ± 1 | 18 ± 2 | 130 ± 9 | 100.0 |
| Factors . | Colony Types . | % of . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | G . | M . | GM . | B . | Eo . | E-Mix . | Total . | Control . |
| None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 |
| SCF | 1 ± 1 | 0 | 0 | 0 | 0 | 0 | 1 ± 1 | 1.0 |
| IL-3 | 0 | 0 | 2 ± 1 | 0 | 8 ± 1 | 0 | 9 ± 0 | 8.7 |
| IL-6 + sIL-6R | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 |
| SCF + IL-3 | 4 ± 2 (NS) | 0 | 5 ± 2 | 0 | 5 ± 1 | 0 | 14 ± 3 | 13.6 |
| SCF + IL-6 + sIL-6R | 2 ± 1 (NS) | 0 | 2 ± 0 | 1 ± 0 (NS) | 0 | 2 ± 1 (NS) | 6 ± 0 | 5.8 |
| IL-3 + IL-6 + sIL-6R | 0 | 0 | 4 ± 1 | 2 ± 0 (NS) | 6 ± 1 | 1 ± 0 (NS) | 12 ± 0 | 11.7 |
| SCF + IL-3 + IL-6 + sIL-6R | 8 ± 1* | 0 | 5 ± 1 | 52 ± 3† | 5 ± 2 | 19 ± 1† | 88 ± 3 | 85.4 |
| CSFs | 11 ± 1 | 1 ± 1 | 18 ± 7 | 55 ± 6 | 2 ± 1 | 18 ± 2 | 130 ± 9 | 100.0 |
Data represent the mean ± SD of duplicate cultures containing 200 CD34+IL-6R− cells/dish. CSFs contained SCF, IL-3, GM-CSF, G-CSF, and Epo.
Abbreviations: G, granulocyte; M, macrophage; GM, granulocyte-macrophage; B, erythroid burst; Eo, eosinophil; E-Mix, erythrocyte-containing mixed colony; NS, not significant.
P < .02.
P < .01.