PCR Primers
| . | . | . | 5′→3′ . |
|---|---|---|---|
| IL-1β | 358 bp | Forward | AGC ACT GAA AGC ATG ATC CGG GAC GTG GAG |
| Reverse | CAG ATA GAT GGG CTC ATA CCA GGG CTT GGC | ||
| IL-2 | 395 bp | Forward | TAC AGG ATG CAA CTC CTG TCT TGC ATT GCA |
| Reverse | GTT GCT GTC TCA TCA GCA TAT TCA CAC ATG | ||
| IL-4 | 365 bp | Forward | CTG CTA GCA TGT GCC GGC AAC TTT GTC CAC |
| Reverse | GAA GTT TTC CAA CGT ACT CTG GTT GGC TTC | ||
| IL-6 | 412 bp | Forward | GAC AAA CAA ATT CGG TAC ATC CTC GAC GGC |
| Reverse | AGT TGT CAT GTC CTG CAG CCA CTG GTT CTG | ||
| IL-10 | 352 bp | Forward | ATG CCC CAA GCT GAG AAC CAA GAC CCA |
| Reverse | TCT CAA GGG GCT GGG TCA GCT ATC CCA | ||
| IFN-γ | 355 bp | Forward | AGT TAT ATC TTG GCT TTT CA |
| Reverse | ACC GAA TAA TTA GTC AGC TT | ||
| TGF-β1 | 336 bp | Forward | GCC CTG GAC ACC AAC TAT TGC |
| Reverse | GCT GCA CTT GCA GGA GCG CAC | ||
| TGF-β2 | 417 bp | Forward | CCA GAA GAC TAT CCT GAG CCC GAG GAA GTC |
| Reverse | ATG TAG CGC TGG GTT GGA GAT GTT AAA TCT | ||
| TNF-α | 123 bp | Forward | TCT CGA ACC CCG AGT GAC AA |
| Reverse | TAT CTC TCA GCT CCA CGC CA | ||
| β-Actin | 406 bp | Forward | AAC CCC AAG GCC AAC CGC GAG AAG ATG ACC |
| Reverse | GGT GAT GAC CTG GCC GTC AGG CAG CTC GTA |
| . | . | . | 5′→3′ . |
|---|---|---|---|
| IL-1β | 358 bp | Forward | AGC ACT GAA AGC ATG ATC CGG GAC GTG GAG |
| Reverse | CAG ATA GAT GGG CTC ATA CCA GGG CTT GGC | ||
| IL-2 | 395 bp | Forward | TAC AGG ATG CAA CTC CTG TCT TGC ATT GCA |
| Reverse | GTT GCT GTC TCA TCA GCA TAT TCA CAC ATG | ||
| IL-4 | 365 bp | Forward | CTG CTA GCA TGT GCC GGC AAC TTT GTC CAC |
| Reverse | GAA GTT TTC CAA CGT ACT CTG GTT GGC TTC | ||
| IL-6 | 412 bp | Forward | GAC AAA CAA ATT CGG TAC ATC CTC GAC GGC |
| Reverse | AGT TGT CAT GTC CTG CAG CCA CTG GTT CTG | ||
| IL-10 | 352 bp | Forward | ATG CCC CAA GCT GAG AAC CAA GAC CCA |
| Reverse | TCT CAA GGG GCT GGG TCA GCT ATC CCA | ||
| IFN-γ | 355 bp | Forward | AGT TAT ATC TTG GCT TTT CA |
| Reverse | ACC GAA TAA TTA GTC AGC TT | ||
| TGF-β1 | 336 bp | Forward | GCC CTG GAC ACC AAC TAT TGC |
| Reverse | GCT GCA CTT GCA GGA GCG CAC | ||
| TGF-β2 | 417 bp | Forward | CCA GAA GAC TAT CCT GAG CCC GAG GAA GTC |
| Reverse | ATG TAG CGC TGG GTT GGA GAT GTT AAA TCT | ||
| TNF-α | 123 bp | Forward | TCT CGA ACC CCG AGT GAC AA |
| Reverse | TAT CTC TCA GCT CCA CGC CA | ||
| β-Actin | 406 bp | Forward | AAC CCC AAG GCC AAC CGC GAG AAG ATG ACC |
| Reverse | GGT GAT GAC CTG GCC GTC AGG CAG CTC GTA |