Colony Formation From Cord Blood CD34+ Cells in the Presence of Various Combinations of FL, IL-6, and sIL-6R in Serum-Containing Culture
| Factor(s) . | No. of Colonies per 500 Cells . | ||||
|---|---|---|---|---|---|
| . | GM . | MK . | B . | Mix . | Total . |
| None | 4 ± 1 | 0† | 0 | 0 | 5 ± 1 |
| FL | 12 ± 2 | 0 | 0 | 0 | 12 ± 2 |
| IL-6 | 6 ± 2 | 1 ± 2 | 0 | 0 | 7 ± 0 |
| sIL-6R | 5 ± 1 | 0† | 0 | 0 | 5 ± 1 |
| IL-6 + sIL-6R | 18 ± 5 | 0 | 8 ± 1 | 7 ± 5 | 33 ± 5* |
| FL + IL-6 | 17 ± 3 | 0† | 0 | 0 | 17 ± 3 |
| FL + sIL-6R | 11 ± 1 | 0 | 0 | 0 | 11 ± 1 |
| FL + IL-6 + sIL-6R | 30 ± 3 | 0 | 11 ± 1 | 17 ± 3 | 58 ± 5* |
| Factor(s) . | No. of Colonies per 500 Cells . | ||||
|---|---|---|---|---|---|
| . | GM . | MK . | B . | Mix . | Total . |
| None | 4 ± 1 | 0† | 0 | 0 | 5 ± 1 |
| FL | 12 ± 2 | 0 | 0 | 0 | 12 ± 2 |
| IL-6 | 6 ± 2 | 1 ± 2 | 0 | 0 | 7 ± 0 |
| sIL-6R | 5 ± 1 | 0† | 0 | 0 | 5 ± 1 |
| IL-6 + sIL-6R | 18 ± 5 | 0 | 8 ± 1 | 7 ± 5 | 33 ± 5* |
| FL + IL-6 | 17 ± 3 | 0† | 0 | 0 | 17 ± 3 |
| FL + sIL-6R | 11 ± 1 | 0 | 0 | 0 | 11 ± 1 |
| FL + IL-6 + sIL-6R | 30 ± 3 | 0 | 11 ± 1 | 17 ± 3 | 58 ± 5* |
500 CD34+ cells were cultured in the presence of various combinations of 100 ng/mL of FL, 100 ng/mL of IL-6, and 1,000 ng/mL of sIL-6R, and the colonies were scored at day 14 of culture. The number of colonies indicates mean ± SD of triplicate cultures.
Colony formation by IL-6/sIL-6R was less than that by FL and IL-6/sIL-6R at P < .01%.
One colony was present in triplicate cultures.