Effects of Varying the Concentration of Anti-gp130 MoAbs on Colony Formation by FL, IL-6, and sIL-6R
| Concentration of MoAbs . | No. of Colonies per 500 Cells . | |||
|---|---|---|---|---|
| (μg/mL) . | GM . | B . | Mix . | Total . |
| 0 | 20 ± 1 | 3 ± 1 | 5 ± 3 | 28 ± 5 |
| 1 | 11 ± 1 | 0 | 0 | 11 ± 1 |
| 10 | 12 ± 2 | 0 | 0 | 12 ± 2 |
| Concentration of MoAbs . | No. of Colonies per 500 Cells . | |||
|---|---|---|---|---|
| (μg/mL) . | GM . | B . | Mix . | Total . |
| 0 | 20 ± 1 | 3 ± 1 | 5 ± 3 | 28 ± 5 |
| 1 | 11 ± 1 | 0 | 0 | 11 ± 1 |
| 10 | 12 ± 2 | 0 | 0 | 12 ± 2 |
Varying concentrations of anti-gp130 MoAbs were added to the serum-containing culture of 500 CD34+ cells with 100 ng/mL of FL, 100 ng/mL of IL-6, and 1,000 ng/mL of sIL-6R, and the colonies were scored at day 14 of culture. The number of colonies indicates mean ± SD of triplicate cultures.