Generation of Various Blood Cells From Cord Blood CD34+ Cells in Serum-Containing Suspension Culture With FL, IL-6, and sIL-6R
| Days in Culture . | Total Cell No. . | No. of Blood Cells . | ||||
|---|---|---|---|---|---|---|
| . | . | G . | M . | MK . | E . | Blast . |
| Day 7 | 2.0 × 104 | 800 | 1,580 | 600 | 0 | 17,020 |
| Day 14 | 10 × 104 | 10,900 | 47,500 | 1,000 | 12,900 | 27,700 |
| Day 21 | 12 × 104 | 28,080 | 66,240 | 840 | 19,920 | 4,920 |
| Days in Culture . | Total Cell No. . | No. of Blood Cells . | ||||
|---|---|---|---|---|---|---|
| . | . | G . | M . | MK . | E . | Blast . |
| Day 7 | 2.0 × 104 | 800 | 1,580 | 600 | 0 | 17,020 |
| Day 14 | 10 × 104 | 10,900 | 47,500 | 1,000 | 12,900 | 27,700 |
| Day 21 | 12 × 104 | 28,080 | 66,240 | 840 | 19,920 | 4,920 |
2,000 CD34+ cells were incubated in serum-containing suspension culture with 100 ng/mL of FL, 100 ng/mL of IL-6, and 1,000 ng/mL of sIL-6R. Every week, total cell numbers were counted, and cytospin preparations were stained with May-Grunwald-Giemsa. Morphology was examined under a light microscope. At least 200 cells were examined.
Abbreviations: G, granulocytes; M, macrophages; E, erythroid cells; Blast, blast cells.