Colony Formation From Cord Blood CD34+ Cells in the Presence of Various Combinations of FL, IL-6, and sIL-6R in Serum-Free Culture
| Factor(s) . | No. of Colonies per 1,000 Cells . | ||||
|---|---|---|---|---|---|
| . | GM . | MK . | B . | Mix . | Total . |
| FL | 17 ± 3 | 0 | 0 | 0 | 17 ± 3 |
| IL-6 + sIL-6R | 0 | 0 | 1 ± 1 | 0 | 1 ± 1 |
| FL + IL-6 + sIL-6R | 87 ± 7 | 0 | 4 ± 3 | 3 ± 1 | 95 ± 10 |
| Factor(s) . | No. of Colonies per 1,000 Cells . | ||||
|---|---|---|---|---|---|
| . | GM . | MK . | B . | Mix . | Total . |
| FL | 17 ± 3 | 0 | 0 | 0 | 17 ± 3 |
| IL-6 + sIL-6R | 0 | 0 | 1 ± 1 | 0 | 1 ± 1 |
| FL + IL-6 + sIL-6R | 87 ± 7 | 0 | 4 ± 3 | 3 ± 1 | 95 ± 10 |
1,000 CD34+ cells were cultured in the presence of various combinations of 100 ng/mL of FL, 100 ng/mL of IL-6, and 1,000 ng/mL of sIL-6R, and the colonies were scored at day 14 of culture. The number of colonies indicates mean ± SD of triplicate cultures.