Cell Surface Expression of L-Selectin, Sialyl-Lewisx, E-Selectin, and CD34 as Measured by Flow Cytometry
| . | TQ1 . | AM-3 . | CSLEX . | BBA2 . | BW 905/151 . | Neg. Control . |
|---|---|---|---|---|---|---|
| PMN | 542 (21) | 480 (10) | nt | nt | nt | 10 (50) |
| 300.19 | 15 (66) | nt | nt | nt | nt | 10 (50) |
| 300.19-L | 340 (164) | nt | nt | nt | nt | 11 (154) |
| NALM-6 | 16 (62) | 116 (433) | nt | nt | nt | 10 (60) |
| NALM-6-L | 1,608 (43) | 115 (443) | nt | nt | nt | 18 (38) |
| HL60 | 16 (62) | nt | nt | nt | nt | 15 (33) |
| HCMEC, unstim. | nt | 16 (56) | 21 (76) | 192 (77) | 105 (286) | 17 (82) |
| HCMEC, TNF-α | nt | 13 (55) | 22 (77) | 4,684 (54) | 88 (277) | 18 (54) |
| . | TQ1 . | AM-3 . | CSLEX . | BBA2 . | BW 905/151 . | Neg. Control . |
|---|---|---|---|---|---|---|
| PMN | 542 (21) | 480 (10) | nt | nt | nt | 10 (50) |
| 300.19 | 15 (66) | nt | nt | nt | nt | 10 (50) |
| 300.19-L | 340 (164) | nt | nt | nt | nt | 11 (154) |
| NALM-6 | 16 (62) | 116 (433) | nt | nt | nt | 10 (60) |
| NALM-6-L | 1,608 (43) | 115 (443) | nt | nt | nt | 18 (38) |
| HL60 | 16 (62) | nt | nt | nt | nt | 15 (33) |
| HCMEC, unstim. | nt | 16 (56) | 21 (76) | 192 (77) | 105 (286) | 17 (82) |
| HCMEC, TNF-α | nt | 13 (55) | 22 (77) | 4,684 (54) | 88 (277) | 18 (54) |
Abbreviations: nt, not tested; unstim, unstimulated.
Measurements of fluorescence intensity obtained with 5 MoAbs (TQ1 for L-selectin, AM3 and CSLEX for sLex, BW 905/151 for CD34, BBA2 for E-selectin). Numbers given represent geometric mean values of 5,000 events on a 4-decade logarithmic scale in 3 independent experiments; coefficients of variation (in %) are given in parentheses.