Inhibition of Human Hematopoietic Progenitors by Exodus
| . | CFU-GM . | BFU-E . | CFU-GEMM . |
|---|---|---|---|
| Medium | 85 ± 3 | 97 ± 4 | 39 ± 3 |
| rHu Exodus (200*) | 43 ± 11 | 43 ± 2 | 19 ± 3 |
| rHu Exodus (100) | 39 ± 3 | 41 ± 2 | 20 ± 2 |
| rHu Exodus (50) | 42 ± 10 | 42 ± 3 | 17 ± 2 |
| rHu Exodus (25) | 41 ± 2 | 50 ± 2 | 20 ± 4 |
| rHu Exodus (12.5) | 51 ± 3 | 70 ± 10 | 27 ± 1 |
| rHu Exodus (6.25) | 64 ± 8 | 87 ± 3 | 32 ± 2 |
| rHu MIP-1 α (100) | 41 ± 2 | 44 ± 3 | 19 ± 2 |
| rHu IL-8 (100) | 42 ± 2 | 44 ± 2 | 19 ± 2 |
| rHu PF-4 (100) | 42 ± 4 | 44 ± 5 | 19 ± 1 |
| rHu RANTES (100) | 81 ± 7 | 99 ± 4 | 37 ± 1 |
| rHu NAP-2 (100) | 83 ± 2 | 93 ± 3 | 39 ± 4 |
| . | CFU-GM . | BFU-E . | CFU-GEMM . |
|---|---|---|---|
| Medium | 85 ± 3 | 97 ± 4 | 39 ± 3 |
| rHu Exodus (200*) | 43 ± 11 | 43 ± 2 | 19 ± 3 |
| rHu Exodus (100) | 39 ± 3 | 41 ± 2 | 20 ± 2 |
| rHu Exodus (50) | 42 ± 10 | 42 ± 3 | 17 ± 2 |
| rHu Exodus (25) | 41 ± 2 | 50 ± 2 | 20 ± 4 |
| rHu Exodus (12.5) | 51 ± 3 | 70 ± 10 | 27 ± 1 |
| rHu Exodus (6.25) | 64 ± 8 | 87 ± 3 | 32 ± 2 |
| rHu MIP-1 α (100) | 41 ± 2 | 44 ± 3 | 19 ± 2 |
| rHu IL-8 (100) | 42 ± 2 | 44 ± 2 | 19 ± 2 |
| rHu PF-4 (100) | 42 ± 4 | 44 ± 5 | 19 ± 1 |
| rHu RANTES (100) | 81 ± 7 | 99 ± 4 | 37 ± 1 |
| rHu NAP-2 (100) | 83 ± 2 | 93 ± 3 | 39 ± 4 |
Colony formation assays of the effect of recombinant human Exodus on the proliferation of hematopoietic progenitors. There was a dose-dependent inhibition of hematopoietic progenitor proliferation by Exodus that was comparable to the inhibitory activities of other β-chemokines known to inhibit the proliferation of hematopoietic progenitors. Experiments were performed twice in triplicate. The values are the mean plus or minus the standard error. There is a statistically significant (Student's t-test) reduction in colony formation at concentrations of Exodus down to 25 ng/mL (P < .005).
Concentrations in ng/mL.