Contributions of Labeled Progenitor Cells to Hematopoiesis
| No. of Transplanted HSC . | Biological Effect of Label . | No. of Simulations with ≥5% Labeled . | No. of Simulations with ≥1% Labeled . | |
|---|---|---|---|---|
| Labeled . | Total . | . | Progenitor Cells at Week 101 . | Progenitor Cells at Week 101 . |
| 1 | 30 | None | 18 | 19 |
| 2 | 30 | None | 29 | 37 |
| 6 | 30 | None | 72 | 79 |
| 2 | 300 | None | 1 | 20 |
| 20 | 300 | None | 58 | 98 |
| 2 | 1,500 | None | 0 | 4 |
| 2 | 300 | 2 × p(HSC replication) | 90 | 90 |
| 2 | 300 | 2 × p(HSC differentiation) | 0 | 0 |
| 2 | 300 | 1/2 × p(HSC differentiation) | 70 | 86 |
| 2 | 300 | 1/2 × p(HSC apoptosis) | 16 | 41 |
| 2 | 300 | 1/2 × p(exhaustion of differentiating clone) | 6 | 27 |
| 2 | 1,500 | 2 × p(HSC replication) | 88 | 89 |
| 2 | 1,500 | 2 × p(HSC differentiation) | 0 | 0 |
| 2 | 1,500 | 1/2 × p(HSC differentiation) | 19 | 67 |
| 2 | 1,500 | 1/2 × p(HSC apoptosis) | 0 | 11 |
| 2 | 1,500 | 1/2 × p(exhaustion of differentiating clone) | 0 | 14 |
| No. of Transplanted HSC . | Biological Effect of Label . | No. of Simulations with ≥5% Labeled . | No. of Simulations with ≥1% Labeled . | |
|---|---|---|---|---|
| Labeled . | Total . | . | Progenitor Cells at Week 101 . | Progenitor Cells at Week 101 . |
| 1 | 30 | None | 18 | 19 |
| 2 | 30 | None | 29 | 37 |
| 6 | 30 | None | 72 | 79 |
| 2 | 300 | None | 1 | 20 |
| 20 | 300 | None | 58 | 98 |
| 2 | 1,500 | None | 0 | 4 |
| 2 | 300 | 2 × p(HSC replication) | 90 | 90 |
| 2 | 300 | 2 × p(HSC differentiation) | 0 | 0 |
| 2 | 300 | 1/2 × p(HSC differentiation) | 70 | 86 |
| 2 | 300 | 1/2 × p(HSC apoptosis) | 16 | 41 |
| 2 | 300 | 1/2 × p(exhaustion of differentiating clone) | 6 | 27 |
| 2 | 1,500 | 2 × p(HSC replication) | 88 | 89 |
| 2 | 1,500 | 2 × p(HSC differentiation) | 0 | 0 |
| 2 | 1,500 | 1/2 × p(HSC differentiation) | 19 | 67 |
| 2 | 1,500 | 1/2 × p(HSC apoptosis) | 0 | 11 |
| 2 | 1,500 | 1/2 × p(exhaustion of differentiating clone) | 0 | 14 |
The number of simulations with ≥1% labeled progenitor cells at week 101 after transplantation with two labeled HSC and 300 total HSC were compared to determine whether a label with a biological effect resulted in a significantly different outcome than that of a neutral label. P values using chi-square tests for equality of proportions were <.0001 for simulations with 2 × p(HSC replication), 2 × p(HSC differentiation), and 1/2 × p(HSC differentiation). P = .002 for 1/2 × p(apoptosis). The P value (.32) for simulations with 1/2 × p(exhaustion of differentiating clone) was not significant.