Absolute Number of LTHC-ICs Detected Among Freshly Isolated and Ex Vivo–Expanded CD34+ Cells
| . | Phenotype . | No. of LTHC-ICs* . | Percent Cells† . |
|---|---|---|---|
| Day 0 | Total CD34+ | 3,750 ± 1,440 | |
| Day 7 | Total CD34+ | 3,481 ± 617 | |
| CD34+ PKH2bright | 1,092 ± 764 | 3.1 ± 2.3 | |
| CD34+ PKH2dim | 2,389 ± 558 | 14.9 ± 1.9 |
| . | Phenotype . | No. of LTHC-ICs* . | Percent Cells† . |
|---|---|---|---|
| Day 0 | Total CD34+ | 3,750 ± 1,440 | |
| Day 7 | Total CD34+ | 3,481 ± 617 | |
| CD34+ PKH2bright | 1,092 ± 764 | 3.1 ± 2.3 | |
| CD34+ PKH2dim | 2,389 ± 558 | 14.9 ± 1.9 |
Simultaneous CD34/PKH2 cell sorting on day 7 was performed as illustrated in Fig 7B. Paired data from 4 BM samples are reported as the mean ± SEM.
Total number of LTHC-ICs normalized per 105 initial CD34+ cells.
Percent cells with indicated phenotype given relative to the total number of cells in culture on day 7.