Table 3. Proliferation Rate of G 0 CD34... | American Society of Hematology

Table 3.

Proliferation Rate of G₀CD34⁺ Cells Isolated Before and After Ex Vivo Expansion

Cell Phenotype	% Cells in S/G₂ + M at Indicated
	Time (h) After Isolation
	0	24	48
G₀CD34⁺	1.45 ± 0.52^3-150	2.61 ± 1.41^†	12.73 ± 3.38^3-150
G₀CD34⁺PKH2^dim	2.55 ± 0.87^3-150	14.24 ± 5.36^†	11.31 ± 2.37^3-150

Cell Phenotype	% Cells in S/G₂ + M at Indicated
	Time (h) After Isolation
	0	24	48
G₀CD34⁺	1.45 ± 0.52^3-150	2.61 ± 1.41^†	12.73 ± 3.38^3-150
G₀CD34⁺PKH2^dim	2.55 ± 0.87^3-150	14.24 ± 5.36^†	11.31 ± 2.37^3-150

Cultures were initiated with freshly isolated BM G₀CD34⁺ cells and G₀CD34⁺PKH2^dim cells at day 7 isolated from paired samples as illustrated in Fig 7. Both cultures were supported with 100 ng/mL each of IL-3, IL-6, and SCF in complete medium. At initiation and at 24-hour intervals, samples were assayed for cell-cycle analysis by the propidium iodide method. Results are expressed as the mean ± SEM, n = 3.

F3-150

P > .05, † P < .05: paired t-tests.

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