Table 5.

Functional Properties of Natural HbS and Recombinant HbS Mutants

HbAdditiveP50n
Natural HbS 0 9 3.1 
 1.2 mmol/L DPG 22 2.8 
 0.5 mmol/L NaCl 22 3.1 
E6V( β)/E121R( β) 0 9 3.1 
 1.2 mmol/L DPG 22 3.1 
 0.5 mmol/L NaCl 21 2.9 
D75Y(α)/E6V( β)/E121R( β) 0 9 3.0 
 1.2 mmol/L DPG 21 2.8 
 0.5 mmol/L NaCl 19 2.8 
D6A(α)/D75Y(α)/E6V( β)/E121R( β) 0 5 2.8 
 1.2 mmol/L DPG 9 2.8 
 0.5 mmol/L NaCl 7 2.8 
HbAdditiveP50n
Natural HbS 0 9 3.1 
 1.2 mmol/L DPG 22 2.8 
 0.5 mmol/L NaCl 22 3.1 
E6V( β)/E121R( β) 0 9 3.1 
 1.2 mmol/L DPG 22 3.1 
 0.5 mmol/L NaCl 21 2.9 
D75Y(α)/E6V( β)/E121R( β) 0 9 3.0 
 1.2 mmol/L DPG 21 2.8 
 0.5 mmol/L NaCl 19 2.8 
D6A(α)/D75Y(α)/E6V( β)/E121R( β) 0 5 2.8 
 1.2 mmol/L DPG 9 2.8 
 0.5 mmol/L NaCl 7 2.8 

The procedures used to calculate the P50 and n values are described in the text, and the Hb concentration for oxygen dissociation curves was 0.6 mmol/L in 50 mmol/L bis-Tris, pH 7.5.

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