Relative Percentages of the Various Colony Types Formed by the MTHC Cells
| . | % Compact . | % Diffuse . | % Mixed . |
|---|---|---|---|
| Nonadherent cells | |||
| MTHC in GM | 79 | 13 | 9 |
| WTKit in GM | 29 | 24 | 46 |
| WTKit in SCF | 45 | 38 | 17 |
| V816Kit in GM | 96 | 1 | 3 |
| V816Kit in SCF | 87 | 6 | 7 |
| V816Kit without factor | 88 | 3 | 9 |
| Adherent cells | |||
| MTHC in GM | 23 | 27 | 50 |
| WTKit in GM | 8 | 17 | 75 |
| WTKit in SCF | 0 | 0 | 100 |
| V816Kit in GM | 81 | 5 | 14 |
| V816Kit in SCF | 65 | 9 | 26 |
| V816Kit without factor | 68 | 5 | 27 |
| . | % Compact . | % Diffuse . | % Mixed . |
|---|---|---|---|
| Nonadherent cells | |||
| MTHC in GM | 79 | 13 | 9 |
| WTKit in GM | 29 | 24 | 46 |
| WTKit in SCF | 45 | 38 | 17 |
| V816Kit in GM | 96 | 1 | 3 |
| V816Kit in SCF | 87 | 6 | 7 |
| V816Kit without factor | 88 | 3 | 9 |
| Adherent cells | |||
| MTHC in GM | 23 | 27 | 50 |
| WTKit in GM | 8 | 17 | 75 |
| WTKit in SCF | 0 | 0 | 100 |
| V816Kit in GM | 81 | 5 | 14 |
| V816Kit in SCF | 65 | 9 | 26 |
| V816Kit without factor | 68 | 5 | 27 |
The colonies formed in methylcellulose (Fig 3) were scored 3 weeks after plating as compact (shown in Fig 4a), diffuse (shown in Fig 4b), and mixed (shown in Fig 4c and d). The number of halo colonies varied depending on the time after plating and were therefore counted as mixed colonies. The numbers above show the percentage of the different colony types of the total number counted.