The Primer of RT-PCR and the Oligonucleotide of In Situ Hybridization
| Table 2A. Upstream and downstream primer for RT-PCR |
| TGF-β (PCR product: 161 bp) |
| Upstream: 5′ GCCCTGGACACCAACTATTGCT 3′ |
| Downstream: 5′ AGGCTCCAAATGTAGGGGCAGG 3′ |
| GM-CSF R α (PCR product: 682 bp) |
| Upstream: 5′ AGAAATCGGATCTGCGAACAGTGGCACC 3′ |
| Downstream: 5′ TCCAGGTACGACAGCTTCTGATAGGTCC 3′ |
| G3PDH (PCR product: 983 bp) |
| Upstream: 5′ TGAAGGTCGGAGTCAACGGATTTGGT 3′ |
| Downstream: 5′ CATGTGGGCCATGAGGTCCACCAC 3′ |
| Table 2B. Oligonucleotide probe for in situ hybridization |
| TGF-β R II |
| 5′ CCCACGTTGCGCAGGTCCTCCCAGCTGATGACATGCCGCG 3′ |
| GM-CSF R α |
| 5′ TGATAGGTCCTGGGCTGTTTCCACCGTACGAGGCAGTGCG 3′ |
| Table 2A. Upstream and downstream primer for RT-PCR |
| TGF-β (PCR product: 161 bp) |
| Upstream: 5′ GCCCTGGACACCAACTATTGCT 3′ |
| Downstream: 5′ AGGCTCCAAATGTAGGGGCAGG 3′ |
| GM-CSF R α (PCR product: 682 bp) |
| Upstream: 5′ AGAAATCGGATCTGCGAACAGTGGCACC 3′ |
| Downstream: 5′ TCCAGGTACGACAGCTTCTGATAGGTCC 3′ |
| G3PDH (PCR product: 983 bp) |
| Upstream: 5′ TGAAGGTCGGAGTCAACGGATTTGGT 3′ |
| Downstream: 5′ CATGTGGGCCATGAGGTCCACCAC 3′ |
| Table 2B. Oligonucleotide probe for in situ hybridization |
| TGF-β R II |
| 5′ CCCACGTTGCGCAGGTCCTCCCAGCTGATGACATGCCGCG 3′ |
| GM-CSF R α |
| 5′ TGATAGGTCCTGGGCTGTTTCCACCGTACGAGGCAGTGCG 3′ |