Table 2A. Upstream and downstream primer for RT-PCR |
TGF-β (PCR product: 161 bp) |
Upstream: 5′ GCCCTGGACACCAACTATTGCT 3′ |
Downstream: 5′ AGGCTCCAAATGTAGGGGCAGG 3′ |
GM-CSF R α (PCR product: 682 bp) |
Upstream: 5′ AGAAATCGGATCTGCGAACAGTGGCACC 3′ |
Downstream: 5′ TCCAGGTACGACAGCTTCTGATAGGTCC 3′ |
G3PDH (PCR product: 983 bp) |
Upstream: 5′ TGAAGGTCGGAGTCAACGGATTTGGT 3′ |
Downstream: 5′ CATGTGGGCCATGAGGTCCACCAC 3′ |
Table 2B. Oligonucleotide probe for in situ hybridization |
TGF-β R II |
5′ CCCACGTTGCGCAGGTCCTCCCAGCTGATGACATGCCGCG 3′ |
GM-CSF R α |
5′ TGATAGGTCCTGGGCTGTTTCCACCGTACGAGGCAGTGCG 3′ |
Table 2A. Upstream and downstream primer for RT-PCR |
TGF-β (PCR product: 161 bp) |
Upstream: 5′ GCCCTGGACACCAACTATTGCT 3′ |
Downstream: 5′ AGGCTCCAAATGTAGGGGCAGG 3′ |
GM-CSF R α (PCR product: 682 bp) |
Upstream: 5′ AGAAATCGGATCTGCGAACAGTGGCACC 3′ |
Downstream: 5′ TCCAGGTACGACAGCTTCTGATAGGTCC 3′ |
G3PDH (PCR product: 983 bp) |
Upstream: 5′ TGAAGGTCGGAGTCAACGGATTTGGT 3′ |
Downstream: 5′ CATGTGGGCCATGAGGTCCACCAC 3′ |
Table 2B. Oligonucleotide probe for in situ hybridization |
TGF-β R II |
5′ CCCACGTTGCGCAGGTCCTCCCAGCTGATGACATGCCGCG 3′ |
GM-CSF R α |
5′ TGATAGGTCCTGGGCTGTTTCCACCGTACGAGGCAGTGCG 3′ |