GPIIIa Oligonucleotides
| Name . | 5′ to 3′ Sequence . | Product Length (bp) . | Comment . |
|---|---|---|---|
| ex0.S | GAGGGGAGGAAGCGCGAGT | −113 to −95 of transcription start site | |
| ex2.A | AAAGGATGCTGTCCTGGCGT | 18 bp 3′ of exon 2 | |
| A.S | CCAAATCTGGTGCTAATGCCT | 157 | 5′ of exon 1 |
| A.A | GAGAAGAAACAAGGTCTGGTAT | 3′ of exon 1 | |
| B.S | TTCTGATTGCTGGACTTCTCTT | 267 | 5′ of exon 2 |
| B.A | TCTCTCCCCATGGCAAAGAGT | 3′ of exon 2 | |
| H.S | CAGTTCAATTCTTGTCTTCTTGT | 209 | 5′ of exon 8 |
| H.A | GCTCCAGGACAAAGGCCCT | 3′ of exon 8 | |
| J.S | CAGCGGGTCCACCTTCCT | 293 | 5′ of exon 10 |
| J.A | CCAGCCTCCCGGCTCTCT | 3′ of exon 10 | |
| L.S | CAGTAGTTGTCTCACTTTTTATT | 229 | 5′ of exon 12 |
| L.A | TGCCTAACATGGTTCTCCCT | 3′ of exon 12 | |
| N.S | AGGAAGTCACTGTAAGATGCT | 153 | 5′ of exon 14 |
| N.A | GGCACAGGCTGATAATGATCT | 3′ of exon 14 | |
| Irrel. | GGAAGCAGACCACGTGGTCTGCTTCC | EMSA competitor |
| Name . | 5′ to 3′ Sequence . | Product Length (bp) . | Comment . |
|---|---|---|---|
| ex0.S | GAGGGGAGGAAGCGCGAGT | −113 to −95 of transcription start site | |
| ex2.A | AAAGGATGCTGTCCTGGCGT | 18 bp 3′ of exon 2 | |
| A.S | CCAAATCTGGTGCTAATGCCT | 157 | 5′ of exon 1 |
| A.A | GAGAAGAAACAAGGTCTGGTAT | 3′ of exon 1 | |
| B.S | TTCTGATTGCTGGACTTCTCTT | 267 | 5′ of exon 2 |
| B.A | TCTCTCCCCATGGCAAAGAGT | 3′ of exon 2 | |
| H.S | CAGTTCAATTCTTGTCTTCTTGT | 209 | 5′ of exon 8 |
| H.A | GCTCCAGGACAAAGGCCCT | 3′ of exon 8 | |
| J.S | CAGCGGGTCCACCTTCCT | 293 | 5′ of exon 10 |
| J.A | CCAGCCTCCCGGCTCTCT | 3′ of exon 10 | |
| L.S | CAGTAGTTGTCTCACTTTTTATT | 229 | 5′ of exon 12 |
| L.A | TGCCTAACATGGTTCTCCCT | 3′ of exon 12 | |
| N.S | AGGAAGTCACTGTAAGATGCT | 153 | 5′ of exon 14 |
| N.A | GGCACAGGCTGATAATGATCT | 3′ of exon 14 | |
| Irrel. | GGAAGCAGACCACGTGGTCTGCTTCC | EMSA competitor |
All primers are complementary to intronic sequence. Product length refers to the size of the PCR product obtained when amplifying the indicated exon.
Abbreviations: S, sense; A, antisense.