Results of the Triple-Probe/Three-Color FISH System Using Normal Samples
| Sample No. . | Cell Type . | Normal . | BCR-ABL+ . | Colocalization of . | Separation of ASS and ABL With no BCR-ABL Colocalization . |
|---|---|---|---|---|---|
| . | . | . | . | ABL-ASS With BCR . | . |
| 1 | BM | 170 (85.0%) | 0 | 23 (11.5%) | 7 (3.5%) |
| 2 | BM | 179 (89.5%) | 0 | 17 (8.5%) | 4 (2.0%) |
| 3 | BM | 173 (86.5%) | 0 | 21 (10.5%) | 6 (3.0%) |
| 4 | BM | 183 (91.5%) | 0 | 13 (6.5%) | 4 (2.0%) |
| 5 | BM | 177 (88.5%) | 0 | 18 (9.0%) | 5 (2.5%) |
| 6 | PBG | 165 (82.5%) | 0 | 27 (13.5%) | 8 (4.0%) |
| Total | 1047 | 0 | 119 | 34 | |
| Mean (%) ±SD | 87.3 ± 3.25 | 9.9 ± 2.43 | 2.8 ± 0.82 |
| Sample No. . | Cell Type . | Normal . | BCR-ABL+ . | Colocalization of . | Separation of ASS and ABL With no BCR-ABL Colocalization . |
|---|---|---|---|---|---|
| . | . | . | . | ABL-ASS With BCR . | . |
| 1 | BM | 170 (85.0%) | 0 | 23 (11.5%) | 7 (3.5%) |
| 2 | BM | 179 (89.5%) | 0 | 17 (8.5%) | 4 (2.0%) |
| 3 | BM | 173 (86.5%) | 0 | 21 (10.5%) | 6 (3.0%) |
| 4 | BM | 183 (91.5%) | 0 | 13 (6.5%) | 4 (2.0%) |
| 5 | BM | 177 (88.5%) | 0 | 18 (9.0%) | 5 (2.5%) |
| 6 | PBG | 165 (82.5%) | 0 | 27 (13.5%) | 8 (4.0%) |
| Total | 1047 | 0 | 119 | 34 | |
| Mean (%) ±SD | 87.3 ± 3.25 | 9.9 ± 2.43 | 2.8 ± 0.82 |
Two hundred cells were analyzed from each sample. Individual cells were categorized as either normal (Fig 4a), BCR-ABL+ (Fig 4b), showing a triple BCR-ABL-ASS colocalization (Fig 4c), or showing separation of ASS and ABL with no BCR-ABL colocalization (Fig 4d). Cells not showing the expected six FISH signals, two from each probe (Fig 4e), were not included in this analysis.
Abbreviations: BM, bone marrow; PBG, peripheral blood granulocyte.