Minimal Cytokine Requirement of Mixed NK Cell Colonies
| Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|
| . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| SF | 0 | 0 | 0 | 4 ± 1 |
| IL-2 | 0 | 0 | 0 | 0 |
| IL-7 | 0 | 0 | 0 | 0 |
| IL-11 | 0 | 0 | 0 | 0 |
| SF, IL-2 | 0 | 0 | 0 | 4 ± 1 |
| SF, IL-7 | 0 | 0 | 2 ± 1 | 4 ± 1 |
| SF, IL-11 | 0 | 0 | 0 | 10 ± 2 |
| IL-2, IL-7 | 0 | 0 | 0 | 0 |
| IL-2, IL-11 | 0 | 0 | 0 | 0 |
| IL-7, IL-11 | 0 | 0 | 0 | 0 |
| SF, IL-2, IL-7 | 0 | 0 ± 1 | 0 ± 1 | 5 ± 2 |
| SF, IL-2, IL-11 | 0 | 0 | 0 ± 1 | 10 ± 2 |
| SF, IL-7, IL-11 | 2 ± 1 | 1 ± 1 | 3 ± 1 | 9 ± 3 |
| IL-2, IL-7, IL-11 | 0 | 0 | 0 ± 1 | 0 |
| SF, IL-2, IL-7, IL-11 | 8 ± 1 | 0 ± 1 | 4 ± 2 | 6 ± 1 |
| Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|
| . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| SF | 0 | 0 | 0 | 4 ± 1 |
| IL-2 | 0 | 0 | 0 | 0 |
| IL-7 | 0 | 0 | 0 | 0 |
| IL-11 | 0 | 0 | 0 | 0 |
| SF, IL-2 | 0 | 0 | 0 | 4 ± 1 |
| SF, IL-7 | 0 | 0 | 2 ± 1 | 4 ± 1 |
| SF, IL-11 | 0 | 0 | 0 | 10 ± 2 |
| IL-2, IL-7 | 0 | 0 | 0 | 0 |
| IL-2, IL-11 | 0 | 0 | 0 | 0 |
| IL-7, IL-11 | 0 | 0 | 0 | 0 |
| SF, IL-2, IL-7 | 0 | 0 ± 1 | 0 ± 1 | 5 ± 2 |
| SF, IL-2, IL-11 | 0 | 0 | 0 ± 1 | 10 ± 2 |
| SF, IL-7, IL-11 | 2 ± 1 | 1 ± 1 | 3 ± 1 | 9 ± 3 |
| IL-2, IL-7, IL-11 | 0 | 0 | 0 ± 1 | 0 |
| SF, IL-2, IL-7, IL-11 | 8 ± 1 | 0 ± 1 | 4 ± 2 | 6 ± 1 |
A total of 50 Sca-1+c-kit+ fetal liver cells were plated in methylcellulose media in the presence of designated cytokines. Colonies were scored after 18 days of incubation. Data represent mean ± SD of quadruplicate cultures.