B Cell and Myeloid Expression of Individual Mixed NK Cell Colonies
| Thy-1+B220− . | B220+ . | Macrophage . | Mast Cell . | No. of Colonies . | |
|---|---|---|---|---|---|
| . | . | . | . | Exp. 1 . | Exp. 2 . |
| + | + | + | + | 4 | 4 |
| + | + | + | 20 | 16 | |
| + | + | 2 | 4 | ||
| + | + | 1 | 0 | ||
| Thy-1+B220− . | B220+ . | Macrophage . | Mast Cell . | No. of Colonies . | |
|---|---|---|---|---|---|
| . | . | . | . | Exp. 1 . | Exp. 2 . |
| + | + | + | + | 4 | 4 |
| + | + | + | 20 | 16 | |
| + | + | 2 | 4 | ||
| + | + | 1 | 0 | ||
Mixed NK cell colonies derived from single cells were harvested from methylcellulose media, divided into two aliquots, and subjected to two-color flow cytometry and May-Grünwald Giemsa staining.